Eismann Lennert, Rodler Severin, Buchner Alexander, Schulz Gerald Bastian, Volz Yannic, Bischoff Robert, Ebner Benedikt, Westhofen Thilo, Casuscelli Jozefina, Waidelich Raphaela, Stief Christian, Schlenker Boris, Ledderose Stephan
Department of Urology, University Hospital Munich, Ludwig-Maximilian-University, 81377 Munich, Germany.
Department of Pathology, University Hospital Munich, Ludwig-Maximilian-University, 80337 Munich, Germany.
Cancers (Basel). 2022 Aug 18;14(16):3999. doi: 10.3390/cancers14163999.
Background: Tumor infiltrating lymphocytes (TILs) are known as important prognostic biomarkers and build the fundament for immunotherapy. However, the presence of TILs and its impact on outcome in pure squamous cell carcinoma (SCC) of the bladder remains uncertain. Methods: Out of 1600 patients undergoing radical cystectomy, 61 patients revealed pure bladder SCC in the final histopathological specimen. Retrospectively, immunohistochemical staining was performed on a subset of TILs (CD3+, CD4+, CD8+, CD20+). Endpoints were overall survival (OS), cancer-specific survival (CSS) and progression-free survival (PFS). The Kaplan−Meier method was used to evaluate survival outcomes. Results: Strong infiltration of CD3+ was found in 27 (44%); of CD4+ in 28 (46%); of CD8+ in 26 (43%); and of CD20+ in 27 tumors (44%). Improved OS was observed for strong CD3+ (p < 0.001); CD4+ (p = 0.045); CD8+ (p = 0.001); and CD20+ infiltration (p < 0.001). Increased rates of PFS were observed for CD3+ (p = 0.025) and CD20+ TILs (p = 0.002). In multivariate analyses, strong CD3+ (HR: 0.163, CI: 0.044−0.614) and strong CD8+ TILs (HR: 0.265, CI: 0.081−0.864) were revealed as predictors for OS and the strong infiltration of CD20+ cells (HR: 0.095, CI: 0.019−0.464) for PFS. Conclusions: These first results of TILs in bladder SCC revealed predictive values of CD3+, CD8+ and CD20+.
肿瘤浸润淋巴细胞（TILs）是重要的预后生物标志物，为免疫治疗奠定了基础。然而，TILs的存在及其对膀胱纯鳞状细胞癌（SCC）预后的影响仍不确定。方法：在1600例行根治性膀胱切除术的患者中，61例患者的最终组织病理学标本显示为单纯膀胱SCC。回顾性地对一部分TILs（CD3 +、CD4 +、CD8 +、CD20 +）进行免疫组化染色。观察终点为总生存期（OS）、癌症特异性生存期（CSS）和无进展生存期（PFS）。采用Kaplan-Meier法评估生存结果。结果：27例（44%）肿瘤中发现CD3 +强浸润；28例（46%）肿瘤中发现CD4 +强浸润；26例（43%）肿瘤中发现CD8 +强浸润；27例（44%）肿瘤中发现CD20 +强浸润。观察到CD3 +（p < 0.001）、CD4 +（p = 0.045）、CD8 +（p = 0.001）和CD20 +浸润（p < 0.001）时OS得到改善。观察到CD3 +（p = 0.025）和CD20 + TILs（p = 0.002）时PFS率增加。在多变量分析中，CD3 +强浸润（HR：0.163，CI：0.044 - 0.614）和CD8 + TILs强浸润（HR：0.265，CI：0.081 - 0.864）被揭示为OS的预测指标，而CD20 +细胞的强浸润（HR：0.095，CI：0.019 - 0.464）为PFS的预测指标。结论：膀胱SCC中TILs的这些初步结果揭示了CD3 +、CD8 +和CD20 +的预测价值。
