Benmansour S, Bonnet J J, Protais P, Costentin J
Neurosci Lett. 1987 Jun 1;77(1):97-102. doi: 10.1016/0304-3940(87)90614-8.
When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.
当钠离子是用于测定大鼠纹状体膜中[3H]GBR 12783特异性结合的孵育培养基中唯一存在的阳离子时，未观察到10至210 mM钠离子之间的钠离子依赖性。在低（10 mM）或高（130 mM）钠离子浓度的培养基中，马吲哚和诺米芬辛以相同的亲和力与[3H]GBR 12783竞争其特异性结合位点。除安咪奈丁外，当钠离子浓度从130 mM降至10 mM时，所有测试的儿茶酚胺摄取抑制剂与[3H]GBR 12783竞争的效力相同。这些数据表明，先前用于多巴胺摄取的氚化抑制剂结合研究的培养基（Tris离子缓冲液和 Krebs-Ringer 培养基）含有在低钠离子浓度下可能对特异性结合产生抑制作用的离子。
