• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

lincRNA-154324 及其毗邻蛋白编码基因在斑马鱼尾部再生中的表达和功能。

The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene in the Caudal Fin Regeneration of Zebrafish.

机构信息

The School of Medical Humanities, Xinxiang Medical University, Xinxiang 453003, China.

Henan International Joint Laboratory of Aquatic Toxicology and Health Protection, College of Life Science, Henan Normal University, Xinxiang 453007, China.

出版信息

Int J Mol Sci. 2022 Aug 11;23(16):8944. doi: 10.3390/ijms23168944.

DOI:10.3390/ijms23168944
PMID:36012210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9409064/
Abstract

Caudal fin regeneration is regulated by a variety of mechanisms, but the role of long non-coding RNA (lncRNA) has rarely been studied. The present study aimed to describe the landscape of lncRNAs during caudal fin regeneration using whole transcriptome sequencing, and then to conduct a functional study on the target lncRNAs using real-time fluorescent quantitative PCR (RT-qPCR), in situ hybridization, and the CRISPR/Cas9 method for lncRNA gene knockout. The results of the transcriptome sequencing showed that a total of 381 lncRNAs were differentially expressed, among which ENSDART00000154324 (lincRNA-154324) was found to be highly related to caudal fin regeneration, and thus it was chosen as the target lncRNA for the subsequent functional study. The results regarding the temporal and spatial expression of lincRNA-154324 and the gene knockout results from CRISPR/Cas9 indicated that lincRNA-154324 is involved in the caudal fin regeneration of zebrafish. Importantly, we serendipitously discovered that the cis correlation coefficient between lincRNA-154324 and its neighboring gene vacuole membrane protein 1 () is extremely high, and they are essential for the process of caudal fin regeneration. Moreover, studies have found that plays an important role in protein secretion, organelle formation, multicellular development, and autophagy. Collectively, our result may provide a framework for the identification and analysis of lncRNAs involved in the regeneration of the zebrafish caudal fin.

摘要

尾部鳍再生受多种机制调控，但长链非编码 RNA（lncRNA）的作用很少被研究。本研究旨在通过全转录组测序描述尾部鳍再生过程中的 lncRNA 图谱，然后使用实时荧光定量 PCR（RT-qPCR）、原位杂交和 CRISPR/Cas9 方法进行 lncRNA 基因敲除，对靶 lncRNA 进行功能研究。转录组测序结果表明，共发现 381 个差异表达的 lncRNA，其中 ENSDART00000154324（lincRNA-154324）与尾部鳍再生高度相关，因此被选为后续功能研究的靶 lncRNA。lincRNA-154324 的时空表达和 CRISPR/Cas9 基因敲除结果表明，lincRNA-154324 参与了斑马鱼的尾部鳍再生。重要的是，我们偶然发现 lincRNA-154324 与其邻近基因液泡膜蛋白 1（）之间的顺式相关系数极高，它们对尾部鳍再生过程至关重要。此外，研究发现在蛋白质分泌、细胞器形成、多细胞发育和自噬中发挥重要作用。总之，我们的结果可能为鉴定和分析参与斑马鱼尾部鳍再生的 lncRNA 提供了一个框架。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/b694d63e6c25/ijms-23-08944-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/4451c83dc4b9/ijms-23-08944-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/70f4cd01caa6/ijms-23-08944-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/1b5bd0d76006/ijms-23-08944-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/118bfb664266/ijms-23-08944-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/f73bf4d4509e/ijms-23-08944-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/0ea4dbdf86a1/ijms-23-08944-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/c26ca39e753b/ijms-23-08944-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/48ba252e0d6b/ijms-23-08944-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/637d1f1fb1c1/ijms-23-08944-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/1b34b90f977b/ijms-23-08944-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/a5a97600a4cb/ijms-23-08944-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/a06a8880b2bc/ijms-23-08944-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/71280f87373c/ijms-23-08944-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/1f86ce59c109/ijms-23-08944-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/b694d63e6c25/ijms-23-08944-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/4451c83dc4b9/ijms-23-08944-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/70f4cd01caa6/ijms-23-08944-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/1b5bd0d76006/ijms-23-08944-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/118bfb664266/ijms-23-08944-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/f73bf4d4509e/ijms-23-08944-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/0ea4dbdf86a1/ijms-23-08944-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/c26ca39e753b/ijms-23-08944-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/48ba252e0d6b/ijms-23-08944-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/637d1f1fb1c1/ijms-23-08944-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/1b34b90f977b/ijms-23-08944-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/a5a97600a4cb/ijms-23-08944-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/a06a8880b2bc/ijms-23-08944-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/71280f87373c/ijms-23-08944-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/1f86ce59c109/ijms-23-08944-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f19/9409064/b694d63e6c25/ijms-23-08944-g015.jpg

相似文献

1
The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene in the Caudal Fin Regeneration of Zebrafish.lincRNA-154324 及其毗邻蛋白编码基因在斑马鱼尾部再生中的表达和功能。
Int J Mol Sci. 2022 Aug 11;23(16):8944. doi: 10.3390/ijms23168944.
2
Functional role of annexins in zebrafish caudal fin regeneration - A gene knockdown approach in regenerating tissue. annexins 在斑马鱼尾鳍再生中的功能作用 - 再生组织中的基因敲低方法。
Biochimie. 2020 Aug;175:125-131. doi: 10.1016/j.biochi.2020.05.014. Epub 2020 Jun 16.
3
Expression analysis of Hsp90α and cytokines in zebrafish caudal fin regeneration.热休克蛋白90α（Hsp90α）和细胞因子在斑马鱼尾鳍再生中的表达分析
Dev Comp Immunol. 2021 Mar;116:103922. doi: 10.1016/j.dci.2020.103922. Epub 2020 Nov 11.
4
Radix Rehmanniae Praeparata promoted zebrafish fin regeneration through aryl hydrocarbon receptor-dependent autophagy.熟地黄通过芳香烃受体依赖性自噬促进斑马鱼鳍再生。
J Ethnopharmacol. 2024 Sep 15;331:118272. doi: 10.1016/j.jep.2024.118272. Epub 2024 May 6.
5
Systematic Identification of Long Noncoding RNAs during Three Key Organogenesis Stages in Zebrafish.系统鉴定斑马鱼三个关键器官发生阶段中的长非编码 RNA。
Int J Mol Sci. 2024 Mar 19;25(6):3440. doi: 10.3390/ijms25063440.
6
Propolis Can Improve Caudal Fin Regeneration in Zebrafish () Induced by The Combined Administration of Alloxan and Glucose.蜂胶可改善由丙烯醛和葡萄糖联合给药诱导的斑马鱼尾部再生。
Zebrafish. 2021 Aug;18(4):274-281. doi: 10.1089/zeb.2020.1969. Epub 2021 Jul 23.
7
Transcriptional profiling of caudal fin regeneration in zebrafish.斑马鱼尾鳍再生的转录谱分析
ScientificWorldJournal. 2006 Jun 2;6 Suppl 1:38-54. doi: 10.1100/tsw.2006.326.
8
Two different transgenes to study gene silencing and re-expression during zebrafish caudal fin and retinal regeneration.用于研究斑马鱼尾鳍和视网膜再生过程中基因沉默和重新表达的两种不同转基因。
ScientificWorldJournal. 2006 Dec 15;6 Suppl 1:65-81. doi: 10.1100/tsw.2006.328.
9
Shp2-Mitogen-Activated Protein Kinase Signaling Drives Proliferation during Zebrafish Embryo Caudal Fin Fold Regeneration.Shp2-丝裂原活化蛋白激酶信号通路在斑马鱼胚胎尾鳍褶再生过程中驱动增殖。
Mol Cell Biol. 2018 Jan 29;38(4). doi: 10.1128/MCB.00515-17. Print 2018 Feb 15.
10
Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.斑马鱼尾鳍再生过程中蛋白酪氨酸磷酸酶的差异氧化。
Sci Rep. 2017 Aug 16;7(1):8460. doi: 10.1038/s41598-017-07109-8.

引用本文的文献

1
Compound 13 Promotes Epidermal Healing in Mouse Fetuses via Activation of AMPK.化合物13通过激活AMPK促进小鼠胎儿的表皮愈合。
Biomedicines. 2023 Mar 27;11(4):1013. doi: 10.3390/biomedicines11041013.

本文引用的文献

1
Regenerative Polarity of the Fin Ray in Zebrafish Caudal Fin and Related Tissue Formation on the Cut Surface.斑马鱼尾鳍鳍条的再生极性及断面上相关组织的形成
J Dev Biol. 2021 Nov 19;9(4):50. doi: 10.3390/jdb9040050.
2
Suppressive effects of valproic acid on caudal fin regeneration in adult zebrafish.丙戊酸对成年斑马鱼尾鳍再生的抑制作用。
Anim Cells Syst (Seoul). 2020 Dec 24;24(6):349-358. doi: 10.1080/19768354.2020.1860126.
3
Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration.钙调神经磷酸酶控制斑马鱼鳍再生中远近轴芽基极性。
Proc Natl Acad Sci U S A. 2021 Jan 12;118(2). doi: 10.1073/pnas.2009539118.
4
An autoregulatory feedback loop of miR-21/VMP1 is responsible for the abnormal expression of miR-21 in colorectal cancer cells.miR-21/VMP1 的自调节反馈回路负责结直肠癌细胞中 miR-21 的异常表达。
Cell Death Dis. 2020 Dec 14;11(12):1067. doi: 10.1038/s41419-020-03265-4.
5
Zebrafish () as a Model for Understanding the Process of Caudal Fin Regeneration.斑马鱼（）作为理解尾鳍再生过程的模型。 你提供的原文中“Zebrafish ()”括号里内容缺失，以上是按完整格式翻译的大致内容。
Zebrafish. 2020 Dec;17(6):359-372. doi: 10.1089/zeb.2020.1926. Epub 2020 Dec 1.
6
Systematic profiling of early regulators during tissue regeneration using zebrafish model.利用斑马鱼模型对组织再生过程中的早期调控因子进行系统分析。
Wound Repair Regen. 2021 Jan;29(1):189-195. doi: 10.1111/wrr.12852. Epub 2020 Aug 12.
7
The role of miR-150 regulates bone cell differentiation and function.miR-150 调节骨细胞分化和功能。
Bone. 2021 Apr;145:115470. doi: 10.1016/j.bone.2020.115470. Epub 2020 Jun 8.
8
Regrowth of zebrafish caudal fin regeneration is determined by the amputated length.斑马鱼尾部再生的程度取决于切除的长度。
Sci Rep. 2020 Jan 20;10(1):649. doi: 10.1038/s41598-020-57533-6.
9
A critical role of VMP1 in lipoprotein secretion.VMP1 在脂蛋白分泌中的关键作用。
Elife. 2019 Sep 17;8:e48834. doi: 10.7554/eLife.48834.
10
Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish.Wilms 瘤 1b 表达定义了一种促再生巨噬细胞亚型，并且是斑马鱼器官再生所必需的。
Cell Rep. 2019 Jul 30;28(5):1296-1306.e6. doi: 10.1016/j.celrep.2019.06.091.