Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Int J Mol Sci. 2022 Aug 12;23(16):9009. doi: 10.3390/ijms23169009.
Glycosylphosphatidylinositol mannosyltransferase I (GPI-MT-I) is an essential glycosyltransferase of glycosylphosphatidylinositol-anchor proteins (GPI-APs) that transfers the first of the four mannoses in GPI-AP precursors, which have multiple functions, including immune response and signal transduction. In this study, the GPI-MT-I gene that regulates GPI-AP biosynthesis in () was characterized for the first time. The open reading frame (ORF) of is 1293 bp and encodes a protein of 430 amino acids that contains a conserved PMT2 superfamily domain. mRNA was widely expressed in the tissues of the Chinese giant salamander. The mRNA expression level of in the spleen, kidney, and muscle cell line (GSM cells) was significantly upregulated post Chinese giant salamander iridovirus (GSIV) infection. The mRNA expression of the virus major capsid protein (MCP) in AdGPI-MT-I-overexpressed cells was significantly reduced. Moreover, a lower level of virus MCP synthesis and gene copying in AdGPI-MT-I-overexpressed cells was confirmed by western blot and ddPCR. These results collectively suggest that GSIV replication in GSM cells was significantly reduced by the overexpression of the AdGPI-MT-I protein, which may contribute to a better understanding of the antiviral mechanism against iridovirus infection.
糖基磷脂酰肌醇甘露糖基转移酶 I（GPI-MT-I）是糖基磷脂酰肌醇锚蛋白（GPI-APs）的必需糖基转移酶，它将 GPI-AP 前体中的四个甘露糖中的第一个转移，GPI-AP 具有多种功能，包括免疫反应和信号转导。在这项研究中，首次对调控中国大鲵 GPI-AP 生物合成的 GPI-MT-I 基因进行了表征。 的开放阅读框（ORF）为 1293bp，编码 430 个氨基酸的蛋白质，包含保守的 PMT2 超家族结构域。 在中华大鲵的组织中广泛表达。 在中国大鲵虹彩病毒（GSIV）感染后，脾脏、肾脏和肌肉细胞系（GSM 细胞）中 的 mRNA 表达水平显著上调。 在过表达 AdGPI-MT-I 的细胞中，病毒主要衣壳蛋白（MCP）的 mRNA 表达显著降低。 此外，通过 Western blot 和 ddPCR 证实，过表达 AdGPI-MT-I 的细胞中病毒 MCP 的合成和基因拷贝数降低。 这些结果共同表明，AdGPI-MT-I 蛋白的过表达显著降低了 GSM 细胞中的 GSIV 复制，这可能有助于更好地理解针对虹彩病毒感染的抗病毒机制。
