Department of General and Transplant Surgery and Nutritional Treatment, Medical University of Lublin, 20-954 Lublin, Poland.
Institute of Quality Assessment and Processing of Animal Products, University of Life Sciences in Lublin, 20-950 Lublin, Poland.
Int J Mol Sci. 2022 Aug 15;23(16):9173. doi: 10.3390/ijms23169173.
Kidney transplantation from a donor with latent BKPyV might be the cause of serious complications, such as BK virus-associated nephropathy. The aim of the study was to determine the prevalence of BKPyV infection in donors after brain death (DBDs), to analyse the molecular variation of BKPyV and to compare clinical and inflammation parameters of DBDs infected with various genotypes of BKPyV. BKPyV was investigated in blood and urine samples of 103 DBDs using PCR followed by sequencing and bioinformatic analysis, and the viral load was assessed by qPCR. Clinical parameters, including cellular markers of inflammation were assessed. The results confirm high prevalence of BKPyV (48%),and genotype IV (49%) over genotype I (43%) and the co-infection with genotypes I and IV in 8.2%. Viral load ranged from 10 to 10 copies/mL, with an average of 1.92 × 10 copies/mL. No specific markers for BKPyV infection were detected among the parameters tested. Infection with genotype I may be associated with the adverse impact on thekidney function, while infection with genotype IV was associated with the anemia Not only the viral load but also the genotype of BKPyV may have an impact on the course of infection.
来自潜伏 BKPyV 供体的肾脏移植可能是导致严重并发症的原因，如 BK 病毒相关性肾病。本研究旨在确定脑死亡（DBD）供体中 BKPyV 感染的流行率，分析 BKPyV 的分子变异，并比较感染不同基因型 BKPyV 的 DBD 的临床和炎症参数。使用 PCR 对 103 例 DBD 的血液和尿液样本进行了 BKPyV 检测，然后进行测序和生物信息学分析，并通过 qPCR 评估病毒载量。评估了临床参数，包括炎症的细胞标志物。结果证实 BKPyV 的高流行率（48%）和基因型 IV（49%）高于基因型 I（43%），以及 8.2%的基因型 I 和 IV 的合并感染。病毒载量范围为 10 至 10 拷贝/ml，平均为 1.92×10 拷贝/ml。在测试的参数中未检测到 BKPyV 感染的特定标志物。与感染基因型 I 可能与肾功能的不良影响相关，而感染基因型 IV 与贫血相关。不仅病毒载量，而且 BKPyV 的基因型也可能对感染过程产生影响。
