Yu Huilin, Jia Wantong, Li Zhongxiang, Gao Chaofeng, Pan Hongyu, Zhang Xianghui
College of Plant Science, Jilin University, Changchun 130062, China.
J Fungi (Basel). 2022 Aug 15;8(8):855. doi: 10.3390/jof8080855.
Sorting nexins are a conserved protein family involved in many cellular processes in fungi, and the function of sorting nexin Snx4 (Atg24) and Snx41 (Atg20) in was not clear. The Δ and Δ mutants were generated by a PCR-based marker method to determine the roles of Snx4 and Snx41 in reproductive development, stress adaption, and virulence in . Compared with the wild-type strain, the Δ and Δ mutants exhibited obvious changes in vegetative growth and in morphology of conidia. In addition, the conidiation, appressorium formation, and virulence of snx4 and snx41 mutants were dramatically reduced. Moreover, Δ and Δ mutants were more sensitive to oxidative stress (menadione and HO), cell wall integrity stress (Congo red and calcofluor white), fungicides, and isothiocyanates. All the phenotypes mentioned above were restored in complemented strains. In addition, ChSnx4 and ChSnx41 were proven to interact with each other through yeast two-hybrid. Taken together, these findings indicated that and were important for fungal growth, asexual development, stress adaption, and virulence in .
分选连接蛋白是一个保守的蛋白质家族，参与真菌中的许多细胞过程，而分选连接蛋白Snx4（Atg24）和Snx41（Atg20）在[具体真菌名称未给出]中的功能尚不清楚。通过基于PCR的标记方法构建了Δ[具体缺失情况未给出]和Δ[具体缺失情况未给出]突变体，以确定Snx4和Snx41在[具体真菌名称未给出]的生殖发育、应激适应和毒力中的作用。与野生型菌株相比，Δ[具体缺失情况未给出]和Δ[具体缺失情况未给出]突变体在营养生长和分生孢子形态上表现出明显变化。此外，snx4和snx41突变体的产孢、附着胞形成和毒力显著降低。而且，Δ[具体缺失情况未给出]和Δ[具体缺失情况未给出]突变体对氧化应激（甲萘醌和过氧化氢）、细胞壁完整性应激（刚果红和荧光增白剂）、杀菌剂和异硫氰酸盐更敏感。上述所有表型在互补菌株中得以恢复。此外，通过酵母双杂交证明ChSnx4和ChSnx41相互作用。综上所述，这些发现表明[具体基因名称未给出]和[具体基因名称未给出]对[具体真菌名称未给出]的真菌生长、无性发育、应激适应和毒力很重要。
