Torres-Garcia Daniel, García Dania, Cano-Lira José F, Gené Josepa
Unitat de Micologia i Microbiologia Ambiental, Facultat de Medicina i Ciències de la Salut and IISPV, Universitat Rovira i Virgili, 43201 Reus, Spain.
J Fungi (Basel). 2022 Aug 17;8(8):868. doi: 10.3390/jof8080868.
Although the is one of the species-richest families in the , research into less-explored substrates can contribute to widening the knowledge of its diversity. In our ongoing survey on culturable from freshwater sediments in Spain, several pleosporacean specimens of taxonomic interest were isolated. Phylogenetic analyses based on five gene markers (ITS, LSU, , 2, and 1) revealed that these fungi represent so far undescribed lineages, which are proposed as two novel genera in the family, i.e., typified by sp. nov., and to accommodate sp. nov. is characterized by the production of phaeodictyospores from apically swollen and darkened conidiogenous cells, the presence of a synanamorph that consists of cylindrical and brown phragmoconidia growing terminally or laterally on hyphae, and by the ability to produce secondary conidia by a microconidiation cycle. is placed phylogenetically distant to any genera in the family and only produces sclerotium-like structures in vitro. The geographic distribution and ecology of and were inferred from metabarcoding data using the GlobalFungi database. The results suggest that is a globally distributed fungus represented by environmental sequences originating primarily from soil samples collected in Australia, Europe, and the USA, whereas is a less common species that has only been found associated with one environmental sequence from an Australian soil sample. The phylogenetic analyses of the environmental ITS1 and ITS2 sequences revealed at least four dark taxa that might be related to and . The phylogeny presented here allows us to resolve the taxonomy of the genus as a member of the .
尽管[具体科名]是[具体范围]中物种最丰富的科之一，但对较少探索的基质进行研究有助于拓宽对其多样性的认识。在我们正在进行的对西班牙淡水沉积物中可培养[具体类群]的调查中，分离出了几个具有分类学意义的格孢腔菌科标本。基于五个基因标记（ITS、LSU、[具体基因名1]、[具体基因名2]和[具体基因名3]）的系统发育分析表明，这些真菌代表了迄今为止未描述的谱系，它们被提议作为该科中的两个新属，即[新属名1]，以[新种名1]为模式种，以及[新属名2]来容纳[新种名2]。[新属名1]的特征是从顶端肿胀和变暗的分生孢子梗细胞产生暗褐色砖格孢子，存在由圆柱形和褐色的隔膜分生孢子组成的合轴分生孢子体，这些分生孢子在菌丝上末端或侧面生长，并且具有通过微分生孢子化循环产生次生分生孢子的能力。[新属名2]在系统发育上与该科中的任何属都相距较远，并且仅在体外产生类似菌核的结构。利用GlobalFungi数据库通过宏条形码数据推断了[新属名1]和[新属名2]的地理分布和生态。结果表明，[新属名1]是一种全球分布的真菌，其环境序列主要来自在澳大利亚、欧洲和美国采集的土壤样本，而[新属名2]是一种较不常见的物种，仅在来自澳大利亚土壤样本的一个环境序列中被发现。对环境ITS1和ITS2序列的系统发育分析揭示了至少四个可能与[新属名1]和[新属名2]相关的深色类群。这里呈现的系统发育关系使我们能够将[具体属名]的分类学解析为[具体科名]的一个成员。
