Muthukumar Vithusan, Shi Lei, Chai Ning, Langenbucher Achim, Becker Sören L, Seitz Berthold, Orosz Erika, Stachon Tanja, Kiderlen Albrecht F, Bischoff Markus, Szentmáry Nóra
Institute for Medical Microbiology and Hygiene, Saarland University, 66421 Homburg, Germany.
Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, 66421 Homburg, Germany.
Microorganisms. 2022 Aug 13;10(8):1642. doi: 10.3390/microorganisms10081642.
keratitis (AK) is a dangerous infectious disease, which is associated with a high risk of blindness for the infected patient, and for which no standard therapy exists thus far. Patients suffering from AK are thus treated, out of necessity, with an off-label therapy, using drugs designed and indicated for other diseases/purposes. Here, we tested the capability of the off-label anti-amoebic drugs chlorhexidine (CH; 0.1%), dibromopropamidine diisethionate (DD; 0.1%), hexamidine diisethionate (HD; 0.1%), miltefosine (MF; 0.0065%), natamycin (NM; 5%), polyhexamethylene biguanide (PHMB; 0.02%), povidone iodine (PVPI; 1%), and propamidine isethionate (PD; 0.1%) to suppress trophozoite formation of and cysts on non-nutrient agar plates. Of the eight off-label anti-amoebic drugs tested, only PVPI allowed for a complete suppression of trophozoite formation by drug-challenged cysts for all four isolates in all five biological replicates. Drugs such as NM, PD, and PHMB repeatedly suppressed trophozoite formation with some, but not all, tested isolates, while other drugs such as CH, DD, and MF failed to exert a relevant effect on the excystation capacities of the tested isolates in most, if not all, of our repetitions. Our findings suggest that pre-testing of the AK isolate with the non-nutrient agar plate assay against the anti-amoebic drug intended for treatment should be performed to confirm that the selected drug is cysticidal for the isolate.
角膜溃疡（AK）是一种危险的传染病，对于受感染患者而言，它伴随着高失明风险，并且迄今为止尚无标准疗法。因此，患有AK的患者出于必要，只能接受一种非标签疗法，即使用针对其他疾病/目的设计和指明的药物进行治疗。在此，我们测试了非标签抗阿米巴药物洗必泰（CH；0.1%）、二溴丙脒二乙磺酸盐（DD；0.1%）、己脒二乙磺酸盐（HD；0.1%）、米替福新（MF；0.0065%）、那他霉素（NM；5%）、聚六亚甲基双胍（PHMB；0.02%）、聚维酮碘（PVPI；1%）和乙磺半胱氨酸丙脒（PD；0.1%）在非营养琼脂平板上抑制 滋养体形成和囊肿的能力。在所测试的八种非标签抗阿米巴药物中，只有PVPI在所有五个生物学重复实验中，对所有四种 分离株经药物处理的囊肿均能完全抑制滋养体形成。诸如NM、PD和PHMB等药物对部分（但并非全部）测试的 分离株反复抑制了滋养体形成，而其他药物如CH、DD和MF在我们大多数（如果不是全部）重复实验中，对所测试的 分离株的脱囊能力未能发挥相关作用。我们的研究结果表明，应使用非营养琼脂平板试验对用于治疗的抗阿米巴药物进行AK分离株的预测试，以确认所选药物对 分离株具有杀囊肿作用。
