Monroy-Nieto Juan, Bowers Jolene R, Montfort Parker, Adame Guillermo, Taverna Constanza Giselle, Yaglom Hayley, Sykes Jane E, Brady Shane, Mochon A Brian, Meyer Wieland, Komatsu Kenneth, Engelthaler David M
Pathogen and Microbiome Division, Translational Genomics Research Institute, Flagstaff, AZ 86005, USA.
Arizona Department of Health Services, Phoenix, AZ 85007, USA.
Microorganisms. 2022 Aug 20;10(8):1681. doi: 10.3390/microorganisms10081681.
Whole-genome sequencing has advanced our understanding of the population structure of the pathogenic species complex Cryptococcus gattii, which has allowed for the phylogenomic specification of previously described major molecular type groupings and novel lineages. Recently, isolates collected in Mexico in the 1960s were determined to be genetically distant from other known molecular types and were classified as VGVI. We sequenced four clinical isolates and one veterinary isolate collected in the southwestern United States and Argentina from 2012 to 2021. Phylogenomic analysis groups these genomes with those of the Mexican VGVI isolates, expanding VGVI into a clade and establishing this molecular type as a clinically important population. These findings also potentially expand the known Cryptococcus ecological range with a previously unrecognized endemic area.
全基因组测序增进了我们对致病性物种复合体加氏隐球菌种群结构的理解，这使得对先前描述的主要分子类型分组和新谱系进行系统基因组学分类成为可能。最近，在20世纪60年代于墨西哥收集的分离株被确定在基因上与其他已知分子类型有很大差异，并被归类为VGVI。我们对2012年至2021年在美国西南部和阿根廷收集的4株临床分离株和1株兽医分离株进行了测序。系统基因组学分析将这些基因组与墨西哥VGVI分离株的基因组归为一组，将VGVI扩展为一个进化枝，并将这种分子类型确立为具有临床重要性的种群。这些发现还可能扩大已知的隐球菌生态范围，发现一个以前未被认识的地方流行区域。
