Yi Yanjie, Luan Pengyu, Wang Kang, Li Guiling, Yin Yanan, Yang Yanhui, Zhang Qingyao, Liu Yang
School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou 450001, China.
Microorganisms. 2022 Aug 20;10(8):1682. doi: 10.3390/microorganisms10081682.
is a worldwide soil-borne pathogenic fungus that significantly infects wheat and causes sharp eyespot in China. However, the biocontrol strains used for the control of are insufficient. In the present study, antagonistic strain B1302 from the rhizosphere of wheat were isolated and identified as based on their morphological, physiological, and biochemical characteristics, and their 16S rDNA sequence. Culture filtrate of strain B1302 had a broad antifungal spectrum. In order to improve the antifungal activity of B1302, response surface methodology (RSM) was used to optimize the culture conditions. The final medium composition and culture conditions were 13.2 g/L of wheat bran, 14.1 g/L of soybean meal, 224 r/min of rotation speed, 7.50 of initial pH, and 1.5 × 10 CFU/mL of inoculation amount at 35 °C for a culture duration of 72 h. B1302 inhibited the hyphae growth of and produced hydrolytic enzymes (protease, chitinase, and glucanase), IAA, and had N-fixing potentiality and P-solubilisation capacity. It can also promote wheat seedling growth in potted plants. The disease incidence and index of wheat seedlings were consistent with the effect of commercial pesticides under treatment with culture filtrate. The biocontrol efficacy of culture filtrate was significant-up to 65.25%. An animal toxicological safety analysis suggested that culture filtrate was safe for use and could be developed into an effective microbial fungicide to control wheat sharp eyespot.
是一种全球范围内的土传致病真菌，在中国严重感染小麦并导致小麦纹枯病。然而，用于防治该病害的生防菌株不足。在本研究中，从小麦根际分离出拮抗菌株B1302，并根据其形态、生理和生化特征以及16S rDNA序列将其鉴定为[具体菌种]。菌株B1302的发酵滤液具有广谱抗菌活性。为了提高B1302的抗菌活性，采用响应面法（RSM）优化培养条件。最终的培养基组成和培养条件为：麦麸13.2 g/L、豆粕14.1 g/L、转速224 r/min、初始pH 7.50、接种量1.5×10 CFU/mL，在35℃下培养72 h。B1302抑制了[病原菌名称]的菌丝生长，产生水解酶（蛋白酶、几丁质酶和葡聚糖酶）、吲哚-3-乙酸（IAA），具有固氮潜力和溶磷能力。它还可以促进盆栽小麦幼苗的生长。在发酵滤液处理下，小麦幼苗的发病率和病情指数与市售农药的效果一致。发酵滤液的生防效果显著，高达65.25%。动物毒理学安全性分析表明，发酵滤液使用安全，可开发成防治小麦纹枯病的有效微生物杀菌剂。
