Rojas-Rechy Moisés H, Gaytán-Morales Félix, Sánchez-Ponce Yessica, Castorena-Villa Iván, López-Martínez Briceida, Parra-Ortega Israel, Escamilla-Núñez María C, Méndez-Tenorio Alfonso, Pompa-Mera Ericka N, Martinez-Ruiz Gustavo U, Fuentes-Pananá Ezequiel M, Morales-Sánchez Abigail
Research Unit in Virology and Cancer, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico.
Postgraduate Program in Biomedicine and Molecular Biotechnology, National Polytechnic Institute, Mexico City 11340, Mexico.
Microorganisms. 2022 Aug 22;10(8):1685. doi: 10.3390/microorganisms10081685.
Infections remain a major cause of morbidity and mortality among hematopoietic stem cell transplant (HSCT) recipients. Unlike Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV), Human Herpesvirus (HHV) 6, HHV7 and HHV8 are not routinely monitored in many centers, especially in the pediatric population of low-medium income countries. We screened EBV, HCMV, HHV6, HHV7 and HHV8 in 412 leukocytes-plasma paired samples from 40 pediatric patients assisted in a tertiary hospital in Mexico. Thirty-two underwent allo-HSCT, whereas eight received auto-HSCT. Overall viral detection frequencies in allo- and auto-HSCT were: EBV = 43.7% and 30.0%, HCMV = 5.0% and 6.7%, HHV6 = 7.9% and 20.0% and HHV7 = 9.7% and 23.3%. HHV8 was not detected in any sample. Interestingly, HHV6 and HHV7 were more frequent in auto-HSCT, and HHV6 was observed in all episodes of multiple detection in auto-HSCT patients. We found EBV DNA in plasma samples, whereas HCMV, HHV6 and HHV7 DNA were predominantly observed in leukocytes, indicative of their expansion in cellular compartments. We also found that IL-1β, IL-2, IL-6 and IL-8 were significantly increased in episodes in which multiple viruses were simultaneously detected, and samples positive for EBV DNA and graft-versus-host disease had a further increase of IL-1β and IL-8. In conclusion, the EBV, HCMV, HHV6 and HHV7 burdens were frequently detected in allo- and auto-HSCT, and their presence associated with systemic inflammation.
感染仍然是造血干细胞移植（HSCT）受者发病和死亡的主要原因。与爱泼斯坦-巴尔病毒（EBV）和人巨细胞病毒（HCMV）不同，人类疱疹病毒（HHV）6、HHV7和HHV8在许多中心并未进行常规监测，尤其是在中低收入国家的儿科人群中。我们对墨西哥一家三级医院收治的40例儿科患者的412份白细胞-血浆配对样本进行了EBV、HCMV、HHV6、HHV7和HHV8筛查。其中32例接受了异基因HSCT，8例接受了自体HSCT。异基因和自体HSCT中总体病毒检测频率分别为：EBV = 43.7%和30.0%，HCMV = 5.0%和6.7%，HHV6 = 7.9%和20.0%，HHV7 = 9.7%和23.3%。所有样本中均未检测到HHV8。有趣的是，HHV6和HHV7在自体HSCT中更为常见，并且在自体HSCT患者的所有多次检测病例中均观察到HHV6。我们在血浆样本中发现了EBV DNA，而HCMV、HHV6和HHV7 DNA主要在白细胞中观察到，这表明它们在细胞区室中扩增。我们还发现，在同时检测到多种病毒的病例中，IL-1β、IL-2、IL-6和IL-8显著升高，并且EBV DNA阳性和移植物抗宿主病的样本中IL-1β和IL-8进一步升高。总之，在异基因和自体HSCT中经常检测到EBV、HCMV、HHV6和HHV7负荷，并且它们的存在与全身炎症相关。
