Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Parkville 3010, Australia.
Mercy Perinatal Research Centre, Melbourne 3084, Australia.
Nutrients. 2022 Aug 11;14(16):3286. doi: 10.3390/nu14163286.
Gestational diabetes mellitus (GDM) and maternal obesity are significant metabolic complications increasingly prevalent in pregnancy. Of major concern, both GDM and maternal obesity can have long-term detrimental impacts on the health of both mother and offspring. Recent research has shown that increased inflammation and oxidative stress are two features central to the pathophysiology of these metabolic conditions. Evidence suggests selenium supplementation may be linked to disease prevention in pregnancy; however, the specific effects of selenium on inflammation and oxidative stress associated with GDM and maternal obesity are unknown. Therefore, this study aimed to investigate the effect of selenium supplementation on an in vitro model of GDM and maternal obesity. Human placental tissue, visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were stimulated with either the bacterial product lipopolysaccharide (LPS) or the pro-inflammatory cytokine TNF-α. Selenium pre-treatment blocked LPS and TNF-α induced mRNA expression and secretion of pro-inflammatory cytokines and chemokines, while increasing anti-inflammatory cytokine and antioxidant mRNA expression in placenta, VAT and SAT. Selenium pre-treatment was also found to inhibit LPS- and TNF-α induced phosphorylation of ERK in placenta, VAT and SAT. These findings indicate that selenium may be able to prevent inflammation and oxidative stress associated with GDM and maternal obesity. Additional in vivo studies are required to identify the efficacy of selenium supplementation in preventing inflammatory pathways activated by GDM and maternal obesity and to elucidate the mechanism involved.
妊娠期糖尿病（GDM）和母体肥胖是妊娠中日益普遍的重要代谢并发症。值得关注的是，GDM 和母体肥胖都会对母婴健康产生长期的不利影响。最近的研究表明，炎症增加和氧化应激是这些代谢疾病发病机制的两个核心特征。有证据表明，硒补充剂可能与妊娠疾病预防有关；然而，硒对 GDM 和母体肥胖相关的炎症和氧化应激的具体影响尚不清楚。因此，本研究旨在探讨硒补充对 GDM 和母体肥胖体外模型的影响。用人胎盘组织、内脏脂肪组织（VAT）和皮下脂肪组织（SAT）分别用细菌产物脂多糖（LPS）或促炎细胞因子 TNF-α刺激。硒预处理阻断了 LPS 和 TNF-α诱导的胎盘、VAT 和 SAT 中促炎细胞因子和趋化因子的 mRNA 表达和分泌，同时增加了抗炎细胞因子和抗氧化剂 mRNA 的表达。还发现硒预处理抑制了 LPS 和 TNF-α诱导的胎盘、VAT 和 SAT 中 ERK 的磷酸化。这些发现表明，硒可能能够预防 GDM 和母体肥胖相关的炎症和氧化应激。需要进行更多的体内研究来确定硒补充预防 GDM 和母体肥胖激活的炎症途径的疗效，并阐明所涉及的机制。
