Ognenovska Samantha, Mukerjee Chinmoy, Sanderson-Smith Martina, Moore Kate H, Mansfield Kylie J
Detrusor Muscle Laboratory, Department of Urogynaecology, University of New South Wales, St. George Hospital, Sydney, NSW 2217, Australia.
Department of Microbiology, St. George Hospital, Sydney, NSW 2217, Australia.
Pathogens. 2022 Aug 17;11(8):926. doi: 10.3390/pathogens11080926.
A recurrent urinary tract infection (UTI) is a common debilitating condition whereby uropathogens are able to survive within the urinary tract. In this study, we aimed to determine if the common uropathogens , , and Group B Streptococcus possessed virulence mechanisms that enable the invasion of urothelial cells. Urothelial cells were isolated from women with detrusor overactivity and recurrent UTIs; the intracellular localisation of the uropathogens was determined by confocal microscopy. Uropathogens were also isolated from women with acute UTIs and their intracellular localisation and virulence mechanisms were examined (yeast agglutination, biofilm formation, and haemolysis). Fluorescent staining and imaging of urothelial cells isolated from women with refractory detrusor overactivity and recurrent UTIs demonstrated that all three uropathogens were capable of intracellular colonisation. Similarly, the bacterial isolates from women with acute UTIs were also seen to intracellularly localise using an in vitro model. All Enterococcus and Streptococcus isolates possessed a haemolytic capacity and displayed a strong biofilm formation whilst yeast cell agglutination was unique to . The expression of virulence mechanisms by these uropathogenic species was observed to correlate with successful urothelial cell invasion. Invasion into the bladder urothelium was seen to be a common characteristic of uropathogens, suggesting that bacterial reservoirs within the bladder contribute to the incidence of recurrent UTIs.
复发性尿路感染（UTI）是一种常见的使人虚弱的病症，尿路病原体能够在尿路内存活。在本研究中，我们旨在确定常见的尿路病原体、和B组链球菌是否具有能够侵入尿道上皮细胞的毒力机制。从患有逼尿肌过度活动症和复发性UTI的女性中分离出尿道上皮细胞；通过共聚焦显微镜确定尿路病原体的细胞内定位。还从患有急性UTI的女性中分离出尿路病原体，并检查其细胞内定位和毒力机制（酵母凝集、生物膜形成和溶血）。对从患有难治性逼尿肌过度活动症和复发性UTI的女性中分离出的尿道上皮细胞进行荧光染色和成像，结果表明所有三种尿路病原体都能够在细胞内定植。同样，使用体外模型也观察到从患有急性UTI的女性中分离出的细菌在细胞内定位。所有肠球菌和链球菌分离株都具有溶血能力，并表现出强烈的生物膜形成，而酵母细胞凝集是独有的特征。观察到这些尿路致病菌种的毒力机制表达与成功侵入尿道上皮细胞相关。侵入膀胱尿道上皮被认为是尿路病原体的一个共同特征，这表明膀胱内的细菌储存库导致了复发性UTI的发生。
