电针对大鼠脑出血损伤的作用机制可能与改善脑铁代谢有关。
Electroacupuncture Reduces Cerebral Hemorrhage Injury in Rats by Improving Cerebral Iron Metabolism.
机构信息
First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China.
Heilongjiang University of Chinese Medicine, China.
出版信息
Mediators Inflamm. 2022 Aug 16;2022:6943438. doi: 10.1155/2022/6943438. eCollection 2022.
OBJECTIVE
To study the effects of electroacupuncture at Baihui and Dazhui points on the expression of hepcidin (Hepc), transferrin (Tf), transferrin receptor (TfR), and ferritin (Ft) in rats with cerebral hemorrhage to provide a theoretical basis for the treatment of cerebral hemorrhage with acupuncture.
METHOD
The model of cerebral hemorrhage in rats was established by autologous blood injection method and treated by electroacupuncture (EA) at the acupoints of Baihui and Dazhui. Hepc siRNA was injected into the lateral ventricle 30 min before model preparation to produce the cerebral hemorrhage model. The modified neurological severity score (mNSS) was used to assess the neurological function, and the total iron content in brain tissue was determined using atomic absorption spectrometry; the expression of Hepc, Ft, Tf, and TfR in perihematoma tissue was detected using immunohistochemistry; the interference efficiency of Hepc siRNA was detected using western blot and reverse transcription polymerase chain reaction (RT-PCR).
RESULTS
The degree of neurological deficit showed a downward trend at 3 days, 7 days, and 14 days, and electroacupuncture significantly reduced the neurological deficit score at each time point ( < 0.01). Regarding total iron content in brain tissue, on the 3rd day, the 7th day, and the 14th day, the iron content of the hematoma tissue after intracerebral hemorrhage was reduced by electroacupuncture ( < 0.01). Regarding immunohistochemical results. Hepc, Ft, Tf, and TfR protein expressions on day 14 were significantly higher after cerebral hemorrhage ( < 0.01). After electroacupuncture, the expression of Hepc, Ft, Tf, and TfR protein was significantly reduced ( < 0.01). Western blot and RT-PCR revealed that the interference efficiency of Hepc siRNA was statistically significant ( < 0.01).
CONCLUSION
Electroacupuncture can reduce neurological severity scores in rats with cerebral hemorrhage and may exert cerebral protective effects by reducing Hepc protein and gene expression; lowering Ft, Tf, and TfR protein expression; and promoting iron metabolism in the brain of rats with cerebral hemorrhage.
目的
研究电针对脑出血大鼠肝脏血红素（Hepc）、转铁蛋白（Tf）、转铁蛋白受体（TfR）和铁蛋白（Ft）表达的影响，为针刺治疗脑出血提供理论依据。
方法
采用自体血注入法制作大鼠脑出血模型，电针百会、大椎穴治疗。在制备模型前 30min 向侧脑室注射 Hepc siRNA 制作脑出血模型。采用改良神经功能缺损评分（mNSS）评价神经功能，原子吸收光谱法测定脑组织总铁含量；免疫组化法检测血肿周围组织 Hepc、Ft、Tf、TfR 表达；Western blot 和逆转录聚合酶链反应（RT-PCR）检测 Hepc siRNA 的干扰效率。
结果
神经功能缺损评分呈下降趋势，在 3 天、7 天和 14 天各时间点电针均能显著降低神经功能缺损评分（ < 0.01）。脑组织总铁含量在脑出血后第 3 天、第 7 天和第 14 天均降低，电针治疗降低了各时间点的铁含量（ < 0.01）。免疫组化结果显示，脑出血后第 14 天血肿组织 Hepc、Ft、Tf、TfR 蛋白表达显著升高（ < 0.01），电针治疗后 Hepc、Ft、Tf、TfR 蛋白表达显著降低（ < 0.01）。Western blot 和 RT-PCR 显示 Hepc siRNA 的干扰效率有统计学意义（ < 0.01）。
结论
电针可降低脑出血大鼠神经功能缺损评分，可能通过降低 Hepc 蛋白和基因表达，降低 Ft、Tf、TfR 蛋白表达，促进脑出血大鼠脑内铁代谢，发挥脑保护作用。
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