Department of Aerospace Hygiene, School of Aerospace Medicine, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Department of Health Education and Management, School of Preventive Medicine, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Undersea Hyperb Med. 2021 Third Quarter;48(3):287-295.
Decompression sickness (DCS) causes serious brain hypoxic-ischemic injury. This experiment was designed to observe whether hyperbaric oxygen (HBO2) pretreatment played a neuroprotective effect in decompression sickness rat models and to explore the mechanism of protective effects.
Sprague-Dawley (SD) male rats were pretreated with HBO2 and then underwent decompression to establish the DCS rat model. Antioxidant capacities were evaluated by detecting peroxides (GPx), superoxide dismutase (SOD), catalase (CAT) activity and malondialdehyde (MDA) content in brains. The levels of metal elements manganese (Mn), zinc (Zn), iron (Fe) and magnesium (Mg) in brain tissues were assessed by flame atomic absorption spectrometry. Necrosis and apoptosis of neurons were assessed by H-E staining and immunohistochemical staining.
HBO2 pretreatment reduced the degree of necrosis and apoptosis in brain tissues of decompression sickness rat models. In addition, HBO2 pretreatment increased GPx, SOD and CAT activities and reduced MDA accumulation. It also increased the content of Mn, Zn, Fe and Mg in brain tissue, which are all related to free radical metabolism.
These results suggested that HBO2 pretreatment has protective effects on brain injury of rats with decompression sickness. The mechanism of the protective effects may be related to reducing oxidative damage by affecting metal elements in vivo.
减压病（DCS）可导致严重的脑缺氧缺血性损伤。本实验旨在观察高压氧（HBO2）预处理对减压病大鼠模型是否具有神经保护作用，并探讨其保护作用的机制。
采用 Sprague-Dawley（SD）雄性大鼠进行 HBO2 预处理，然后进行减压以建立 DCS 大鼠模型。通过检测脑内过氧化物（GPx）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量来评估抗氧化能力。采用火焰原子吸收光谱法评估脑组织中金属元素锰（Mn）、锌（Zn）、铁（Fe）和镁（Mg）的含量。通过 H-E 染色和免疫组织化学染色评估神经元的坏死和凋亡情况。
HBO2 预处理可减轻减压病大鼠模型脑组织的坏死和凋亡程度。此外，HBO2 预处理可提高 GPx、SOD 和 CAT 的活性，减少 MDA 的积累。它还增加了脑组织中 Mn、Zn、Fe 和 Mg 的含量，这些都与自由基代谢有关。
这些结果表明，HBO2 预处理对减压病大鼠的脑损伤具有保护作用。保护作用的机制可能与通过影响体内金属元素来减轻氧化损伤有关。
