β-CD-牡荆素微球的制备及其对 SW480 细胞增殖的影响。

Preparation of β-CD-Vitexin Microspheres and their Effects on SW480 Cell Proliferation.

机构信息

College of Life Science, Zaozhuang University, Zaozhuang 277160, China.

Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution, Xinyang Normal University, Xinyang 464000, China.

出版信息

Curr Drug Deliv. 2023;20(4):433-440. doi: 10.2174/1567201819666220825090426.

DOI:10.2174/1567201819666220825090426

PMID:36017865

Abstract

OBJECTIVE

In order to overcome the insolution and low bioavailability of the vitexin in vivo, β-cyclodextrin-vitexin (β-CD-vitexin) microspheres were prepared, and their effects on the proliferation of SW480 cells were observed.

METHODS

Scanning electron microscopy, ultraviolet spectrum, Fourier transform infrared spectroscopy, and release rate analysis identified the formation of β-CD-vitexin microspheres. MTT assay detected the effect of β-CD-vitexin microspheres on tumor cell proliferation at 6, 12, 24, and 48 h. Fluorescence microscopy and flow cytometry were used to observe the effect of β-CD-vitexin microspheres on the apoptosis of SW480 cells. The mRNA expression of the p53 gene was measured by qPCR.

RESULTS

β-CD-vitexin microspheres were successfully prepared. SW480 cell proliferation was inhibited by 0.1, 0.2, and 0.4 mg/mL of β-CD-vitexin microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to cell apoptosis caused by the upregulated expression of p53 gene.

CONCLUSION

The preparation of β-CD-vitexin sustained release microspheres is feasible, and β-CDvitexin microspheres have potential anti-colorectal cancer value.

摘要

目的

为克服染料木黄酮在体内的溶解问题和低生物利用度，制备β-环糊精-染料木黄酮（β-CD-染料木黄酮）微球，并观察其对 SW480 细胞增殖的影响。

方法

扫描电子显微镜、紫外光谱、傅里叶变换红外光谱和释放率分析鉴定β-CD-染料木黄酮微球的形成。MTT 法检测 6、12、24 和 48 h 时β-CD-染料木黄酮微球对肿瘤细胞增殖的影响。荧光显微镜和流式细胞术观察β-CD-染料木黄酮微球对 SW480 细胞凋亡的影响。采用 qPCR 检测 p53 基因的 mRNA 表达。