[Effect of the nutrient medium components on the formation of growth inhibition zones of the test microbe in determining the biological activity of mycoheptin].

The effect of the nutrient medium components on the diffusion properties of mycoheptin and the growth of Candida utilis as the test-microbe was studied. It was found that the content of various amounts of sodium and potassium chlorides, disubstituted sodium phosphate, glucose, yeast extract, peptone, agar-agar and the value of pH in the medium had a significant effect on the size of the inhibition growth zones of the test-culture, clearance of their margin and the angle of the dose-response curve. The nutrient medium composition considered to be optimal for determination of the mychoheptin activity is proposed.