Fu Kaiqiang, Sun Yuning, Wang Junbo, Cao Rongfeng
Qingdao Agricultural University, Shandong, Qingdao 266109, PR China.
Qingdao Agricultural University, Shandong, Qingdao 266109, PR China; Qingdao Hengxing University of Science and Technology, Shandong, Qingdao 266100, PR China.
Res Vet Sci. 2022 Dec 10;151:149-155. doi: 10.1016/j.rvsc.2022.08.008. Epub 2022 Aug 10.
Mastitis is the most prevalent disease in dairy cows worldwide. Evidence has emerged that oxidative stress plays a crucial role in the development of mastitis. This study aimed to investigate the antioxidative effects of tanshinone IIa (Tan IIa) on LPS-induced oxidative stress in dairy cow mammary epithelial cells (CMECs).
We examined the levels of ROS and MDA in LPS-treated CMECs after supplementation with Tan IIa using detection kits and found that Tan IIa significantly inhibited the upregulation of these factors. In addition, we also found that Tan IIa significantly reversed the decrease in mitochondrial membrane potential induced by LPS. Moreover, Tan IIa improved the activities of antioxidant enzymes, which were decreased by LPS. Finally, we examined the probable pathway in which Tan IIa exerted its antioxidant effects using qPCR and western blotting and found that Tan IIa significantly activated the Keap1/Nrf2 signalling pathway.
These results suggest that Tan IIa might become a possible therapeutic agent for the treatment of dairy cow mastitis by weakening oxidative stress induced by LPS in CMECs.
乳腺炎是全球奶牛中最普遍的疾病。有证据表明氧化应激在乳腺炎的发展中起关键作用。本研究旨在探讨丹参酮IIa（Tan IIa）对脂多糖（LPS）诱导的奶牛乳腺上皮细胞（CMECs）氧化应激的抗氧化作用。
我们使用检测试剂盒检测了添加Tan IIa后LPS处理的CMECs中活性氧（ROS）和丙二醛（MDA）的水平，发现Tan IIa显著抑制了这些因子的上调。此外，我们还发现Tan IIa显著逆转了LPS诱导的线粒体膜电位下降。此外，Tan IIa提高了被LPS降低的抗氧化酶活性。最后，我们使用定量聚合酶链反应（qPCR）和蛋白质免疫印迹法检测了Tan IIa发挥抗氧化作用的可能途径，发现Tan IIa显著激活了Kelch样环氧氯丙烷相关蛋白1（Keap1）/核因子E2相关因子2（Nrf2）信号通路。
这些结果表明，Tan IIa可能通过减弱LPS在CMECs中诱导的氧化应激而成为治疗奶牛乳腺炎的一种可能的治疗药物。
