Key Laboratory for Liquid-Solid Structural Evolution & Processing of Materials (Ministry of Education), School of Materials Science and Engineering, Shandong University, Jinan, Shandong 250061, PR China.
Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong, PR China.
J Colloid Interface Sci. 2022 Dec 15;628(Pt B):595-604. doi: 10.1016/j.jcis.2022.08.086. Epub 2022 Aug 17.
The widespread use of antibiotics leads to the increasing drug resistance of bacteria and poses a threat to human health. Therefore, there is an urgent need to develop new antibacterial strategies. Herein, based on the good photothermal properties of Copper sulfide (CuS) nanoparticles under near infrared (NIR) laser, we developed a NIR-Ⅱ window triple-mode synergetic antibacterial cCuS (cationic CuS) @Vancomycin (Van) nanoplatform. In the proposed nanoplatform, the positive charge on the surface makes cCuS@Van nanoplatform show better bacterial uptake and membrane damage; vancomycin induces chemical sterilization and provides a targeting effect to the nanoplatform; combined with the strong photothermal effect and deep tissue penetration at the excitation of 1064 nm laser, cCuS@Van nanoplatform can effectively kill bacterial. The photothermal conversion efficiency of the nanoplatform can reach 49.12 % and in vitro experiments show a sterilizing rate of more than 99.5 % to staphylococcus aureus (S. aureus) at the concentration of 3.0 μM, which also demonstrated the synergistic effect of cCuS@Van nanoplatform. In addition, low cytotoxicity to human cells conforms the good biocompatibility of the as-prepared cCuS@Van nanoplatform, which endows it a good application prospect in the field of antibacterial, such as wound healing and implant sterilization.
抗生素的广泛使用导致细菌的耐药性不断增加，对人类健康构成威胁。因此，迫切需要开发新的抗菌策略。在此，基于硫化铜（CuS）纳米粒子在近红外（NIR）激光下良好的光热性能，我们开发了一种 NIR-Ⅱ 窗三模式协同抗菌 cCuS（阳离子 CuS）@万古霉素（Van）纳米平台。在提出的纳米平台中，表面上的正电荷使 cCuS@Van 纳米平台表现出更好的细菌摄取和膜损伤；万古霉素诱导化学灭菌并为纳米平台提供靶向作用；结合 1064nm 激光激发下的强光热效应和深组织穿透性，cCuS@Van 纳米平台能够有效杀死细菌。该纳米平台的光热转换效率可达 49.12%，体外实验表明，在 3.0μM 的浓度下，对金黄色葡萄球菌（S. aureus）的杀菌率超过 99.5%，这也证明了 cCuS@Van 纳米平台的协同作用。此外，对人细胞的低细胞毒性符合所制备的 cCuS@Van 纳米平台的良好生物相容性，这使其在抗菌领域（如伤口愈合和植入物消毒）具有良好的应用前景。
