Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 5;284:121771. doi: 10.1016/j.saa.2022.121771. Epub 2022 Aug 19.
A highly sensitive and selective sensor for the quantitative assay of inorganic pyrophosphatase (PPase) activity was developed based on a fluorescence "turn-off" strategy. Carbon quantum dots@Cu(II)-based metal-organic framework nanotubes (CQDs@Cu-MOF) with length less than 300 nm and width less than 20 nm were synthesized. CQDs in the nanotubes exhibited weak fluorescence owing to static quenching. The coordination reaction between pyrophosphate ion (PPi) and Cu(II) decomposed CQDs@Cu-MOF and led to the release of CQDs, of which the fluorescence recovered. In the presence of PPase, the hydrolysis of PPi generated phosphate ion (Pi). CQDs@Cu-MOF remained their structural stability and the fluorescence turned off. The fluorescence intensity difference of the mixture of CQDs@Cu-MOF and PPi in the absence and presence of PPase (-ΔF) was proportional to the PPase concentration from 0.1 to 5 mU mL and that from 5 to 50 mU mL, and a limit of detection at 0.03 mU mL was obtained. PPase activity in human serum was analyzed using the proposed fluorescence sensor and the recovery values were found to vary from 95.0% to 104 %.
基于荧光“关闭”策略，开发了一种用于定量测定无机焦磷酸酶（PPase）活性的高灵敏度和选择性传感器。合成了长度小于 300nm、宽度小于 20nm 的碳量子点@Cu(II)-基于金属有机骨架纳米管（CQDs@Cu-MOF）。由于静态猝灭，纳米管中的 CQDs 表现出较弱的荧光。焦磷酸盐离子（PPi）与 Cu(II)之间的配位反应分解 CQDs@Cu-MOF 并导致 CQDs 的释放，其荧光恢复。在存在 PPase 的情况下，PPi 的水解产生磷酸根离子（Pi）。CQDs@Cu-MOF 保持其结构稳定性，荧光关闭。在没有和存在 PPase 的情况下，CQDs@Cu-MOF 和 PPi 的混合物的荧光强度差异（-ΔF）与 0.1 至 5mU mL 范围内的 PPase 浓度成正比，与 5 至 50mU mL 范围内的 PPase 浓度成正比，并且在 0.03mU mL 时获得了检测限。使用所提出的荧光传感器分析了人血清中的 PPase 活性，发现回收率在 95.0%到 104%之间变化。
