Medina-De la Garza C E, Brattig N W, Tischendorf F W
Trop Med Parasitol. 1987 Mar;38(1):53-4.
Microfilariae of O. volvulus were purified from dissected nodules by centrifugation on discontinuous Percoll density gradients. Nodules were used freshly or after cryopreservation in liquid nitrogen. Highly purified and motile microfilariae were recovered from a layer of a density of 1.090 g/ml. The isolated microfilariae retained their motility up to 3 days when cultured in vitro.
通过在不连续的 Percoll 密度梯度上离心，从解剖的结节中纯化出旋盘尾丝虫微丝蚴。结节可新鲜使用或在液氮中冷冻保存后使用。从密度为 1.090 g/ml 的一层中回收高度纯化且具有运动能力的微丝蚴。分离出的微丝蚴在体外培养时可保持其运动能力长达 3 天。
