Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Changsha, 410128, China.
Commun Biol. 2022 Aug 26;5(1):881. doi: 10.1038/s42003-022-03850-7.
Chlorops oryzae is a pest of rice that has caused severe damage to crops in major rice-growing areas in recent years. We generated a 447.60 Mb high-quality chromosome-level genome with contig and scaffold N50 values of 1.17 Mb and 117.57 Mb, respectively. Hi-C analysis anchored 93.22% scaffolds to 4 chromosomes. The relatively high expression level of Heat Shock Proteins (HSPs) and antioxidant genes in response to thermal stress suggests these genes may play a role in the environmental adaptability of C. oryzae. The identification of multiple pathways that regulate reproductive development (juvenile hormone, 20-hydroxyecdsone, and insulin signaling pathways) provides evidence that these pathways also play an important role in vitellogenesis and thus insect population maintenance. These findings identify possible reasons for the increased frequency of outbreaks of C. oryzae in recent years. Our chromosome-level genome assembly may provide a basis for further genetic studies of C. oryzae, and promote the development of novel, sustainable strategies to control this pest.
稻绿蝽是近年来在主要水稻种植区对作物造成严重危害的一种水稻害虫。我们生成了一个高质量的 447.60Mb 染色体水平基因组，其 contig 和 scaffold N50 值分别为 1.17Mb 和 117.57Mb。Hi-C 分析将 93.22%的支架锚定到 4 条染色体上。热激蛋白 (HSPs) 和抗氧化基因在应对热胁迫时的相对高表达水平表明，这些基因可能在稻绿蝽的环境适应性中发挥作用。鉴定出多个调节生殖发育的途径（保幼激素、20-羟蜕皮酮和胰岛素信号通路）为这些途径在卵黄生成中也发挥重要作用，从而维持昆虫种群提供了证据。这些发现为近年来稻绿蝽爆发频率增加的可能原因提供了依据。我们的染色体水平基因组组装可能为进一步研究稻绿蝽的遗传机制提供基础，并促进开发新的、可持续的控制这一害虫的策略。
