年龄和性别对 SARS-CoV-2 疫苗接种后刺突蛋白抗体状态的影响，以及过去感染对大阪医护人员的影响。
Age- and sex-based changes in spike protein antibody status after SARS-CoV-2 vaccination and effect of past-infection in healthcare workers in Osaka.
机构信息
Department of Clinical Practice, Yao Municipal Hospital, 1-3-1 Ryuge-cho, Yao, Osaka, 581-0069, Japan.
出版信息
BMC Infect Dis. 2022 Aug 26;22(1):709. doi: 10.1186/s12879-022-07695-7.
OBJECTIVE
We aimed to compare the changes in SARS-CoV-2 spike protein antibody titres based on age group and sex using paired blood sampling after vaccination in association with the presence of nucleocapsid protein antibody.
METHODS
All participants were healthcare workers at Yao Municipal Hospital in Osaka who voluntarily provided peripheral blood samples (n = 636, men/women 151/485, mean age 45 years). We investigated the serial changes in SARS-CoV-2 spike protein antibody titres at 1 and 7 months after the second vaccination regarding their relationship with sex and age group. At 7 months, we also examined anti-nucleocapsid assays. Antibody titres were shown as logarithmic values and the differences were assessed using a paired or unpaired student's t-test as appropriate.
RESULTS
Among participants younger than 30 years, the antibody titres of spike protein were significantly higher in women one (p = 0.005) and seven (p = 0.038) months after vaccination. However, among those aged 30-49 years, the antibody titres were not different between the sexes at either follow-up time point. In contrast, among those aged 50-59 years, between-sex differences in antibody titres were observed only at 7 months, which was associated with a significant reduction in men. A significant negative correlation was observed between the antibody titres for spike protein at both time points in participants with positive nucleocapsid protein antibody at 7 months (r = - 0.467, p = 0.043), although a significant positive correlation was observed in those with negative results (r = 0.645, p < 0.001), CONCLUSIONS: Between-sex differences in SARS-CoV-2 spike protein antibody titres by paired blood sampling at different time points after vaccination depended on age group. The presence of nucleocapsid protein antibody was associated with changes in spike protein antibody titres after vaccination.
目的
通过接种疫苗后配对采血，比较基于年龄组和性别的 SARS-CoV-2 刺突蛋白抗体滴度的变化，并结合核衣壳蛋白抗体的存在情况。
方法
所有参与者均为大阪市立耀医院的医护人员，他们自愿提供外周血样本（n=636，男性/女性 151/485，平均年龄 45 岁）。我们调查了接种第二针疫苗后 1 个月和 7 个月时 SARS-CoV-2 刺突蛋白抗体滴度的连续变化，并研究了其与性别和年龄组的关系。在 7 个月时，我们还检查了抗核衣壳检测。抗体滴度显示为对数，并使用配对或非配对学生 t 检验进行评估。
结果
在年龄小于 30 岁的参与者中，女性在接种疫苗后 1 个月（p=0.005）和 7 个月（p=0.038）时的刺突蛋白抗体滴度显著更高。然而，在 30-49 岁的参与者中，在两个随访时间点，性别之间的抗体滴度没有差异。相比之下，在 50-59 岁的参与者中，只有在 7 个月时才观察到性别之间的抗体滴度差异，这与男性的显著下降有关。在 7 个月时，抗核衣壳蛋白抗体阳性的参与者的刺突蛋白抗体在两个时间点的抗体滴度均呈显著负相关（r=-0.467，p=0.043），而抗核衣壳蛋白抗体阴性的参与者的抗体滴度呈显著正相关（r=0.645，p<0.001）。
结论
接种疫苗后不同时间点配对采血的 SARS-CoV-2 刺突蛋白抗体滴度的性别差异取决于年龄组。核衣壳蛋白抗体的存在与接种疫苗后刺突蛋白抗体滴度的变化有关。
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