Charmo Research Center, Charmo University, Chamchamal 46023, Kurdistan Region-Iraq.
Medical Laboratory Analysis Department, Cihan University Sulaymaniyah, Sulaymaniyah 46001, Kurdistan Region, Iraq.
Cell Mol Biol (Noisy-le-grand). 2022 May 31;68(5):33-46. doi: 10.14715/cmb/2022.68.5.5.
Although the production of the secondary metabolite is frequently restricted, methods to regulate and optimize their synthesis are extremely beneficial. The current study proposes to enhance the production of antibiofilm metabolite in Streptomyces cellulosae (S. cellulosae). It was isolated from soil by growing on Gause's media and identified by colony morphology and genomic sequencing of 16S rDNA. Antibacterial and antibiofilm activities of the isolates were screened against a series of pathogenic bacteria by agar plug diffusion and 96 well microtiter plate methods, respectively. Physiological regulation of the bacterial bioactivity against biofilm formation was monitored under different cultural conditions. The isolated Streptomyces sequence analysis of the 16S rDNA was 100% identical to the sequence of S. cellulosae strain NBRC 13027. Physical (temperature and pH) and chemical (carbon, nitrogen, and minerals) culture medium factors have shown variable impacts on the growth and bioactive substances of S. cellulosae. Moreover, results of simple linear regression and correlation suggested that most of the physiological regulations with the highest response (r2= 0.85-0.99; p<0.01) and linearly (r= 0.88-0.99; p<0.01) were correlated between microbial biomass and crude extract. Lastly, under different culture growth conditions, biofilm inhibition was tested against Pseudomonas aeruginosa (P. aeruginosa). The physiological regulation results exhibited that 1 μg/mL of the extract was the most efficient concentration against biofilm formation in P. aeruginosa while 3 μg/mL is an effective bactericidal dose against P. aeruginosa. We concluded that S. cellulosae can produce antibacterial and antibiofilm metabolites. Physiological regulation is considered a powerful tool that can be used for increasing the biosynthesis of the active metabolites and biomass.
尽管次生代谢产物的产生经常受到限制，但调节和优化其合成的方法是非常有益的。本研究旨在提高纤维素链霉菌（S. cellulosae）中抗生物膜代谢物的产量。该菌是通过在 Gause 培养基上生长从土壤中分离出来的，通过菌落形态和 16S rDNA 基因组测序进行鉴定。通过琼脂塞扩散和 96 孔微量滴定板方法分别筛选分离物对一系列致病菌的抑菌和抗生物膜活性。在不同的培养条件下监测细菌生物活性对生物膜形成的生理调节。分离出的链霉菌 16S rDNA 序列分析与 S. cellulosae NBRC 13027 菌株的序列完全一致。物理（温度和 pH 值）和化学（碳、氮和矿物质）培养基因素对纤维素链霉菌的生长和生物活性物质表现出不同的影响。此外，简单线性回归和相关性的结果表明，大多数生理调节与微生物生物量和粗提取物之间具有最高响应（r2=0.85-0.99；p<0.01）和线性（r=0.88-0.99；p<0.01）关系。最后，在不同的培养生长条件下，测试了对铜绿假单胞菌（P. aeruginosa）的生物膜抑制作用。生理调节结果表明，1μg/mL 的提取物是抑制铜绿假单胞菌生物膜形成的最有效浓度，而 3μg/mL 是对铜绿假单胞菌有效的杀菌剂量。我们得出结论，纤维素链霉菌可以产生抗菌和抗生物膜代谢物。生理调节被认为是一种强大的工具，可以用于增加活性代谢物和生物量的生物合成。
