Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Institute of Pathology, Clinical Center Osnabrueck, Osnabrueck, Germany.
Ann Diagn Pathol. 2022 Oct;60:152029. doi: 10.1016/j.anndiagpath.2022.152029. Epub 2022 Aug 20.
Cytokeratin 10 (CK10) is a type I acidic low molecular weight cytokeratin which is mainly expressed in keratinizing squamous epithelium of the skin. Variable levels of CK10 protein have been described in squamous carcinomas of different sites and in some other epithelial neoplasms. To comprehensively determine the prevalence of CK10 expression in normal and neoplastic tissues, a tissue microarray containing 11,021 samples from 131 different tumor types and subtypes was analyzed by immunohistochemistry. CK10 immunostaining was detectable in 41 (31.3 %) of 131 tumor categories, including 18 (13.7 %) tumor types with at least one strongly positive case. The highest rate of positive staining was found in squamous cell carcinomas from various sites of origin (positive in 18.6 %-66.1 %) and in Warthin tumors of salivary glands (47.8 %), followed by various tumor entities known to potentially exhibit areas with squamous cell differentiation such as teratomas (33.3 %), basal cell carcinomas of the skin (14.3 %), adenosquamous carcinomas of the cervix (11.1 %), and several categories of urothelial neoplasms (3.1 %-16.8 %). In a combined analysis of 956 squamous cell carcinomas from 11 different sites of origin, reduced CK10 staining was linked to high grade (p < 0.0001) and advanced stage (p = 0.0015) but unrelated to HPV infection. However, CK10 staining was not statistically related to grade (p = 0.1509) and recurrence-free (p = 0.5247) or overall survival (p = 0.5082) in 176 cervical squamous cell carcinomas. In the urinary bladder, CK10 staining occurred more commonly in muscle-invasive (17.7 %) than in non-invasive urothelial carcinomas (4.0 %-6.0 %; p < 0.0001). In summary, our data corroborate a role of CK10 as a suitable marker for mature, keratinizing squamous cell differentiation in epithelial tissues. CK10 immunohistochemistry may thus be instrumental for a more objective evaluation of the clinical significance of focal squamous differentiation in cancer.
细胞角蛋白 10（CK10）是一种 I 型酸性低分子量细胞角蛋白，主要表达于皮肤的角化鳞状上皮。在不同部位的鳞状细胞癌和一些其他上皮性肿瘤中，CK10 蛋白的水平有不同程度的描述。为了全面确定 CK10 在正常和肿瘤组织中的表达率，通过免疫组织化学分析了包含 131 种不同肿瘤类型和亚型的 11021 个样本的组织微阵列。在 131 种肿瘤类型中的 41 种（31.3%）中可检测到 CK10 免疫染色，包括 18 种（13.7%）至少有一个强阳性病例的肿瘤类型。阳性染色率最高的是来自不同起源部位的鳞状细胞癌（阳性率为 18.6%-66.1%）和唾液腺的沃辛瘤（47.8%），其次是已知具有潜在鳞状细胞分化区域的各种肿瘤实体，如畸胎瘤（33.3%）、皮肤基底细胞癌（14.3%）、宫颈的腺鳞癌（11.1%）和几类尿路上皮肿瘤（3.1%-16.8%）。在对来自 11 个不同起源部位的 956 例鳞状细胞癌的综合分析中，CK10 染色减少与高分级（p<0.0001）和晚期（p=0.0015）相关，但与 HPV 感染无关。然而，在 176 例宫颈鳞状细胞癌中，CK10 染色与分级（p=0.1509）、无复发生存（p=0.5247）或总生存（p=0.5082）无关。在膀胱中，CK10 染色在肌层浸润性（17.7%）比非浸润性尿路上皮癌（4.0%-6.0%）更常见（p<0.0001）。总之，我们的数据证实了 CK10 作为上皮组织中成熟角化鳞状细胞分化的合适标志物的作用。因此，CK10 免疫组化可能有助于更客观地评估癌症中局灶性鳞状分化的临床意义。
