Pôle de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, Brasília DF, Brazil.
J Photochem Photobiol B. 2022 Sep;234:112546. doi: 10.1016/j.jphotobiol.2022.112546. Epub 2022 Aug 20.
The field of photodynamic therapy (PDT) for treating various malignant neoplasms has been given researchers' attention due to its ability to be a selective and minimally invasive cancer therapy strategy. The possibility of tumor cell infection and hence high recurrence rates in cancer patients tends to restrict autologous transplantation. So, the photodynamic tissue purging process, which consists of selective photoinactivation of the malignant cells in the graft, is defined as a compromising strategy to purify contaminated tissues before transplantation. In this strategy, the direct malignant cells' death results from the reactive oxygen species (ROS) generation through the activation of a photosensitizer (PS) by light exposure in the presence of oxygen. Since new PS generations can effectively penetrate the tissue, PDT could be an ideal ex vivo tissue purging protocol that eradicates cancer cells derived from various malignancies. The challenge is that the applied pharmacologic ex vivo tissue purging should efficiently induce tumor cells with minor influence on normal tissue cells. This review aims to provide an overview of the current status of the most effective PDT strategies and PS development concerning their potential application in ex vivo purging before hematopoietic stem cell or ovarian tissue transplantation.
由于光动力疗法 (PDT) 具有选择性和微创性癌症治疗策略的能力，因此，研究人员一直关注该疗法在治疗各种恶性肿瘤中的应用。癌症患者肿瘤细胞感染的可能性以及因此导致的高复发率往往限制了自体移植。因此，光动力组织清除过程被定义为一种妥协策略，即在移植前通过选择性光灭活移植物中的恶性细胞来净化受污染的组织。在该策略中，通过在氧存在下用光激活光敏剂 (PS) 产生活性氧物种 (ROS)，直接导致恶性细胞死亡。由于新一代 PS 可以有效地穿透组织，因此 PDT 可能是一种理想的离体组织清除方案，可以消除源自各种恶性肿瘤的癌细胞。挑战在于，应用药理学离体组织清除应有效地诱导肿瘤细胞，而对正常组织细胞的影响较小。本综述旨在概述最有效的 PDT 策略和 PS 开发的现状，以及它们在造血干细胞或卵巢组织移植前离体清除中的潜在应用。
