大蹼铃蟾脂多糖刺激皮肤分泌物中存在铃蟾激肽原M片段，并具有脂多糖中和活性。
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity.
作者信息
Zhang Baiyu, Yin Saige, Guo Caifen, Gao Zhenhua, Li Tonghai, Lee Wenhui, Shen Jihong, Yang Xinwang
机构信息
First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.
Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan 650500, China.
出版信息
J Immunol Methods. 2022 Oct;509:113343. doi: 10.1016/j.jim.2022.113343. Epub 2022 Aug 25.
Lipopolysaccharide (LPS) is a major pathogen-associated pattern molecule that can initiate lethal sepsis. Bioactive peptides in amphibian skin secretions, especially antimicrobial peptides, are essential components of the host immune system and help fight the microbial invasion. In this study, two peptides: peptide 1 (KINRKGPRPPG) and peptide 2 (INRKGPRPPG) were isolated, from skin secretions of the Chinese red belly frog (Bombina maxima). After stimulation with LPS, peptide 1 showed direct LPS-binding activity, low cytotoxicity, immunoregulatory functions in vitro, and neutralizing LPS effects in animal models. Thus, natural peptide 1 exhibits potential as an ideal candidate against LPS.
脂多糖（LPS）是一种主要的病原体相关模式分子，可引发致死性败血症。两栖动物皮肤分泌物中的生物活性肽，尤其是抗菌肽，是宿主免疫系统的重要组成部分，有助于抵抗微生物入侵。在本研究中，从中国红腹铃蟾（Bombina maxima）的皮肤分泌物中分离出两种肽：肽1（KINRKGPRPPG）和肽2（INRKGPRPPG）。用LPS刺激后，肽1表现出直接的LPS结合活性、低细胞毒性、体外免疫调节功能以及在动物模型中中和LPS作用。因此，天然肽1展现出作为抗LPS理想候选物的潜力。
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