Ko Jaeeun, Kim Jaehwan, Choi Yang-Kyu, Nahm Sang-Soep, Kim Jayon, Seo Sun-Min, Seo Jin-Seok, Lee Woojong, Chung Weon Kuu, Eom Kidong
Department of Veterinary Medical Imaging, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.
Front Vet Sci. 2022 Aug 12;9:839467. doi: 10.3389/fvets.2022.839467. eCollection 2022.
This study aimed to evaluate the clinical safety and validate the radiomitigative effect of KMRC011, against radiation-induced oral mucositis in beagle dogs. Clinical safety was evaluated by assessing tolerability, complete blood tests, and plasma biochemistry after drug administration. The radiomitigative effect of KMRC011 was evaluated macropathologically and histopathologically after inducing oral mucositis iatrogenically using 20 Gy irradiation. The plasma concentration of interleukin-6 was measured enzyme-linked immunosorbent assay, as a biomarker of KMRC011 bioreactivity. Decreased tolerability, increased neutrophil count, hepatic enzyme concentration, C-reactive protein concentration, and interleukin-6 concentration after the administration was observed and ceased within 24 h without additional treatment. Although all animals included in the present study developed severe mucositis in the late course of the study, animals administered KMRC011 showed less erythema, ulcer, inflammatory infiltration. These results suggest that KMRC011 may be used as an adjuvant for radiotherapy without severe adverse effects, especially during short-term radiotherapy, such as hypofractionated radiotherapy or stereotactic radiotherapy.
本研究旨在评估KMRC011对比格犬放射性口腔黏膜炎的临床安全性并验证其辐射缓解效果。通过给药后评估耐受性、全血细胞检测和血浆生化指标来评价临床安全性。使用20 Gy照射医源性诱导口腔黏膜炎后，从大体病理学和组织病理学方面评估KMRC011的辐射缓解效果。采用酶联免疫吸附测定法测量白细胞介素-6的血浆浓度，作为KMRC011生物活性的生物标志物。给药后观察到耐受性降低、中性粒细胞计数增加、肝酶浓度、C反应蛋白浓度和白细胞介素-6浓度升高，且在未进行额外治疗的情况下24小时内恢复正常。尽管本研究纳入的所有动物在研究后期均出现了严重的黏膜炎，但给予KMRC011的动物出现的红斑、溃疡、炎症浸润较少。这些结果表明，KMRC011可作为放疗的辅助药物，且无严重不良反应，尤其是在短期放疗（如大分割放疗或立体定向放疗）期间。
