Shah Dawood, Iqbal Arshad, Alshehri Fahad S, Ullah Aman, Ali Gowhar, Muhammad Tahir, Ullah Rahim, Sewell Robert D E, Althobaiti Yusuf S
Department of Botany, Islamia College Peshawar, Peshawar, Pakistan.
Department of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia.
J Inflamm Res. 2022 Aug 22;15:4785-4802. doi: 10.2147/JIR.S376242. eCollection 2022.
Alzheimer's disease (AD) is a neurodegenerative disorder that is more prevalent in the elderly. There is extensive literature on using species against central nervous system disorders, although has not been investigated for any neuroprotective potential. The purpose of the study was to elucidate the ameliorative effect of ethyl acetate (ASEE) and butanol (ASB) extracts from the bark of on memory deficits and cognitive dysfunction in scopolamine- or diazepam-induced amnesia in mice.
The phytochemical constituents of the extracts of were determined by GC-MS and the in vitro anticholinesterase plus antioxidant activities were also evaluated. Anti-amnesic effects were determined employing the open field test, elevated plus maze (EPM), Morris water maze (MWM), and Y-maze paradigms.
The in vitro cholinesterase assays disclosed a concentration-dependent inhibition of both and with IC values of 28.48 and 44.86 µg/mL for the ASEE extract and 32.04 and 50.26 µg/mL for the ASB extract against and respectively. DPPH and HO antioxidant assays revealed respective IC values for the ASEE extract of 28.04 and 59.84 µg/mL, plus 32.77 and 64.65 µg/mL for ASB extract. The findings revealed that both extracts possessed substantial antioxidant properties. Furthermore, these fractions restored scopolamine- and diazepam-induced memory deficits in a dose-dependent manner, as observed by a significant decrease in the transfer latency in EPM, reduction in escape latency, added time spent in the target quadrant in the MWM, and elevated spontaneous alternation behavior (SAB) in the Y-maze test.
The ameliorative effect of on scopolamine- and diazepam-induced amnesia can be attributed to antioxidant and anticholinesterase activity. Consequently, the use of might be exploited in the alleviation of oxidative stress and the management of AD.
阿尔茨海默病（AD）是一种在老年人中更为普遍的神经退行性疾病。关于使用[植物名称]对抗中枢神经系统疾病已有大量文献报道，尽管尚未对其任何神经保护潜力进行研究。本研究的目的是阐明从[植物名称]树皮中提取的乙酸乙酯提取物（ASEE）和丁醇提取物（ASB）对东莨菪碱或地西泮诱导的小鼠失忆模型中记忆缺陷和认知功能障碍的改善作用。
通过气相色谱 - 质谱联用（GC - MS）测定提取物的植物化学成分，并评估其体外抗胆碱酯酶和抗氧化活性。采用旷场试验、高架十字迷宫（EPM）、莫里斯水迷宫（MWM）和Y迷宫范式来确定抗失忆作用。
体外胆碱酯酶测定显示，ASEE提取物对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）的抑制呈浓度依赖性，IC值分别为28.48和44.86μg/mL；ASB提取物对AChE和BuChE的IC值分别为32.04和50.26μg/mL。二苯基苦味酰基自由基（DPPH）和羟基自由基（HO）抗氧化试验显示，ASEE提取物的IC值分别为28.04和59.84μg/mL，ASB提取物的IC值分别为32.77和64.65μg/mL。结果表明两种提取物都具有显著的抗氧化特性。此外，这些提取物能以剂量依赖性方式恢复东莨菪碱和地西泮诱导的记忆缺陷，表现为EPM中转移潜伏期显著缩短、MWM中逃避潜伏期缩短、在目标象限的停留时间增加以及Y迷宫试验中自发交替行为（SAB）增强。
[植物名称]对东莨菪碱和地西泮诱导的失忆的改善作用可归因于其抗氧化和抗胆碱酯酶活性。因此，[植物名称]的使用可能有助于减轻氧化应激和管理AD。
