Binzhou Medical University, Laishan District, Yantai, 264003 Shandong, China.
Biomed Res Int. 2022 Aug 17;2022:3768880. doi: 10.1155/2022/3768880. eCollection 2022.
Dihuang Yinzi, as a classical Chinese medicine prescription, plays an important role for the treatment of ischemic stroke. Gut microbiota play a functional role for the expression of proinflammatory cytokines and anti-inflammatory cytokines, which further affect central nervous system and change brain function. Our research confirmed that Dihuang Yinzi can exert brain protection by inhibiting inflammatory reaction. Dihuang Yinzi can significantly decrease the contents of interleukin-6 (IL-6), tumor necrosis factor- (TNF-), and interleukin-17 (IL-17) in brain, serum, and colon tissues and increase the contents of transforming growth factor- (TGF-) and interleukin-10 (IL-10) in cerebral ischemia-reperfusion model rats. The results of 16s rRNA high-throughput sequencing showed that Dihuang Yinzi had a significant effect on microbiome in rats. The firmicutes, bacteroidetes, and proteobacteria were dominant in Dihuang Yinzi group. The content of firmicutes increased with the increase of dosage of Dihuang Yinzi. Especially, the content of actinomycetes in the high-dose group was higher than other groups. At the genus level, the number of bacteroides in the antibiotic groups was significantly higher than that in the other treatment groups. The results suggest that Dihuang Yinzi may play important roles in treatment of ischemic stroke by regulating the gut microbiota and the inflammatory reaction in the colon tissues, serum, and brain of the model rats, to verify the scientific nature of this prescription in relieving brain inflammatory reaction and brain injury by this way and to reveal the brain-gut related mechanism of Dihuang Yinzi in treating ischemic stroke.
地黄引子作为一种经典的中药方剂，在治疗缺血性中风方面发挥着重要作用。肠道微生物群在促炎细胞因子和抗炎细胞因子的表达中发挥功能作用，进一步影响中枢神经系统并改变大脑功能。我们的研究证实，地黄引子可以通过抑制炎症反应发挥脑保护作用。地黄引子可显著降低脑、血清和结肠组织中白细胞介素-6（IL-6）、肿瘤坏死因子-（TNF-）和白细胞介素-17（IL-17）的含量，增加脑缺血再灌注模型大鼠转化生长因子-（TGF-）和白细胞介素-10（IL-10）的含量。16s rRNA 高通量测序结果表明，地黄引子对大鼠微生物群有显著影响。厚壁菌门、拟杆菌门和变形菌门在地黄引子组中占优势。随着地黄引子剂量的增加，厚壁菌门的含量增加。特别是，高剂量组放线菌的含量高于其他组。在属水平上，抗生素组中拟杆菌的数量明显高于其他治疗组。结果表明，地黄引子可能通过调节肠道微生物群和模型大鼠结肠组织、血清和大脑中的炎症反应，对缺血性中风发挥治疗作用，验证了该方剂通过这种方式缓解大脑炎症反应和脑损伤的科学性，并揭示了地黄引子治疗缺血性中风的脑-肠相关机制。
