Khan Md Muzahedul I, Yousuf Mohammad A, Ahamed Parbhej, Alauddin Mohammad, Tonu Nusrat T
Department of Chemistry, Khulna University of Engineering and Technology, Khulna 9203, Bangladesh.
Department of Theoretical and Computational Chemistry, University of Dhaka, Dhaka 1000, Bangladesh.
ACS Omega. 2022 Aug 11;7(33):29391-29405. doi: 10.1021/acsomega.2c03651. eCollection 2022 Aug 23.
In this work, an HB pencil electrode (HBPE) was electrochemically modified by amino acids (AAs) glycine (GLY) and aspartic acid (ASA) and designated as GLY-HB and ASA-HB electrodes. They were used in the detection of dihydroxybenzene isomers (DHBIs) such as hydroquinone (HQ), catechol (CC), and resorcinol (RS), by cyclic voltammetry (CV), and by differential pulse voltammetry. HBPE was characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. These three electrodes showed a linear relationship of current with concentration of DHBIs, and the electrochemical processes were diffusion controlled in all cases. In simultaneous detection, the limit of detection, based on signal-to-noise ratio (S/N = 3), for HQ, CC, and RS was 12.473, 16.132, and 25.25 μM, respectively, at bare HBPE; 5.498, 7.119, and 14.794 μM, respectively, at GLY-HB; and 22.459, 25.478, and 38.303 μM, respectively, at ASA-HB. The sensitivity for HQ, CC, and RS was 470.481, 363.781, and 232.416 μA/mM/cm, respectively, at bare HBPE; 364.785, 282.712, and 135.560 μA/mM/cm, respectively, at GLY-HB; and 374.483, 330.108, and 219.574, respectively, at ASA-HB. The interference studies clarified the suitability and reliability of the electrodes for the detection of HQ, CC, and RS in an environmental system. Real sample analysis was done using tap water, and the proposed electrodes expressed recovery with high reproducibility. Meanwhile, these three electrodes have excellent sensitivity and selectivity, which can be used as a promising technique for the detection of DHBIs simultaneously.
在本研究中，用氨基酸（AAs）甘氨酸（GLY）和天冬氨酸（ASA）对HB铅笔电极（HBPE）进行电化学修饰，分别命名为GLY-HB电极和ASA-HB电极。采用循环伏安法（CV）和差分脉冲伏安法，利用这些电极检测对苯二酚（HQ）、邻苯二酚（CC）和间苯二酚（RS）等二羟基苯异构体（DHBIs）。通过扫描电子显微镜和能量色散X射线光谱对HBPE进行表征。这三种电极的电流与DHBIs浓度呈线性关系，且在所有情况下电化学过程均受扩散控制。在同时检测时，基于信噪比（S/N = 3），裸HBPE上HQ、CC和RS的检测限分别为12.473、16.132和25.25 μM；GLY-HB上分别为5.498、7.119和14.794 μM；ASA-HB上分别为22.459、25.478和38.303 μM。裸HBPE上HQ、CC和RS的灵敏度分别为470.481、363.781和232.416 μA/ mM/cm；GLY-HB上分别为364.785、282.712和135.560 μA/ mM/cm；ASA-HB上分别为374.483、330.108和219.574。干扰研究明确了这些电极在环境系统中检测HQ、CC和RS的适用性和可靠性。使用自来水进行实际样品分析，所提出的电极具有高重现性的回收率。同时，这三种电极具有出色的灵敏度和选择性，可作为一种有前景的同时检测DHBIs的技术。
