羟酰基辅酶A脱氢酶可能是2型糖尿病早期血管内皮损伤的靶分子。
HADH may be the target molecule of early vascular endothelial impairment in T2DM.
作者信息
Ye Haowen, Wang Ruxin, Wei Jinjing, Wang Ying, Wang Lihong, Zhang Xiaofang
机构信息
Department of Endocrinology and Metabolism, First Affiliated Hospital of Jinan University, Guangzhou, China.
Department Clinical Experimental Center, First Affiliated Hospital of Jinan University, Guangzhou, China.
出版信息
Front Cardiovasc Med. 2022 Aug 10;9:963916. doi: 10.3389/fcvm.2022.963916. eCollection 2022.
BACKGROUND
Type 2 diabetes mellitus (T2DM) will significantly increase the risk of atherosclerosis (AS). Vascular endothelial cell dysfunction (VECD) is the foundation of AS. Early identification and intervention of VECD caused by T2DM can help us effectively delay or even suppress the occurrence of AS.
METHODS
We downloaded the gene expression profiles from the Gene Expression Omnibus (GEO). The differential expression genes (DEGs) were identified in R software and weighted gene co-expression network analysis (WGCNA) was performed to further screen the target genes. In addition, we used the receiver operating characteristic curve (ROC curve) to verify the diagnostic efficiency of target genes. Finally, target genes were validated by quantitative polymerase chain reaction (qPCR).
RESULTS
Four target genes (, and ) were discovered in early vascular endothelial impairment caused by T2DM through differential expression analysis and WGCNA. The ROC curve of target genes showed that had the best diagnostic efficacy in VECD and AS. qPCR showed that the mRNA level expression of and were decreased in human coronary artery endothelial cells (HCAECs) treated with high glucose and palmitic acid.
CONCLUSION
may be the target gene in early VECD caused by T2DM.
背景
2型糖尿病（T2DM）会显著增加动脉粥样硬化（AS）的风险。血管内皮细胞功能障碍（VECD）是AS的基础。早期识别和干预由T2DM引起的VECD有助于我们有效延缓甚至抑制AS的发生。
方法
我们从基因表达综合数据库（GEO）下载基因表达谱。在R软件中鉴定差异表达基因（DEGs），并进行加权基因共表达网络分析（WGCNA）以进一步筛选靶基因。此外，我们使用受试者工作特征曲线（ROC曲线）验证靶基因的诊断效率。最后，通过定量聚合酶链反应（qPCR）验证靶基因。
结果
通过差异表达分析和WGCNA在由T2DM引起的早期血管内皮损伤中发现了四个靶基因（、和）。靶基因的ROC曲线表明在VECD和AS中具有最佳诊断效能。qPCR显示在高糖和棕榈酸处理的人冠状动脉内皮细胞（HCAECs）中，和的mRNA水平表达降低。
结论
可能是由T2DM引起的早期VECD中的靶基因。
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