ACOT7 可保护表皮干细胞免受脂质过氧化。
ACOT7 protects epidermal stem cells against lipid peroxidation.
机构信息
Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People's Republic of China.
Jinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound Repair, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, People's Republic of China.
出版信息
In Vitro Cell Dev Biol Anim. 2022 Aug;58(7):549-557. doi: 10.1007/s11626-022-00703-9. Epub 2022 Aug 29.
Epidermal stem cells (ESCs) are critical for skin regeneration and repair. Previous studies have shown that ESCs are susceptible to oxidative stress, which in turn leads to lipid peroxidation and affects skin repair. Our study aims to explore how ESCs resist lipid peroxidation. By performing proteomics analysis, we found that the expression of Acyl-CoA thioesterase 7 (ACOT7) was positively correlated with the concentration of transferrin. Overexpression adenovirus vectors of ACOT7 were constructed and transfected into ESCs. Levels of lipid peroxidation by flow cytometry, cell viabilities, and MDA levels were measured. The results revealed that ACOT7 could inhibit lipid peroxidation, reduce the level of malondialdehyde (MDA), and improve the survival rate of ESCs induced by HO, Erastin, and RSL3. Our data suggest that ACOT7 has an effect on protecting ESCs against iron-dependent lipid peroxidation.
表皮干细胞（ESCs）对于皮肤再生和修复至关重要。先前的研究表明，ESCs 容易受到氧化应激的影响，这反过来又导致脂质过氧化，并影响皮肤修复。我们的研究旨在探讨 ESCs 如何抵抗脂质过氧化。通过蛋白质组学分析，我们发现酰基辅酶 A 硫酯酶 7（ACOT7）的表达与转铁蛋白的浓度呈正相关。构建了 ACOT7 的过表达腺病毒载体，并转染到 ESCs 中。通过流式细胞术测量脂质过氧化水平、细胞活力和 MDA 水平。结果表明，ACOT7 可以抑制脂质过氧化，降低丙二醛（MDA）水平，并提高 HO、Erastin 和 RSL3 诱导的 ESCs 的存活率。我们的数据表明，ACOT7 对保护 ESCs 免受铁依赖性脂质过氧化具有作用。
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