Effects of tumor necrosis factor on tumor cell plasma membrane permeability.

Ehrlich ascites tumor cells incubated with rabbit tumor necrosis factor (TNF)-serum showed a temperature-dependent functional impairment of the plasma membrane as reflected by permeability changes. Trypan blue inclusion and Ca2+-transport were considerably increased at hyperthermia temperature. This characteristic of the TNF-plasma membrane interaction may explain the cytotoxicity of TNF.