SP1/miR-92a-1-5p/SOCS5：猫泛白细胞减少症病毒复制中的新调控轴。
SP1/miR-92a-1-5p/SOCS5: A novel regulatory axis in feline panleukopenia virus replication.
机构信息
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
出版信息
Vet Microbiol. 2022 Oct;273:109549. doi: 10.1016/j.vetmic.2022.109549. Epub 2022 Aug 27.
MicroRNAs (miRNAs) are vital post-transcriptional regulators that participate in host-pathogen interactions by modulating the expression of cellular factors. Previous studies have demonstrated that feline panleukopenia virus (FPV) alters miRNA expression levels within host cells. However, the relationship between FPV replication and host miRNAs remains unclear. Here, we demonstrated that FPV infection significantly altered cellular miR-92a-1-5p expression in F81 cells by upregulating the expression of specificity protein 1 (SP1). Furthermore, we observed that miR-92a-1-5p enhanced interferon (IFN-α/β) expression by targeting the suppressors of cytokine signaling 5 (SOCS5) that negatively regulates NF-κB signaling and inhibits FPV replication in host cells. These findings revealed that miR-92a-1-5p plays a crucial role in host defense against FPV infection.
微小 RNA（miRNA）是重要的转录后调控因子，通过调节细胞因子的表达参与宿主-病原体相互作用。先前的研究表明，猫泛白细胞减少症病毒（FPV）改变了宿主细胞内 miRNA 的表达水平。然而，FPV 复制与宿主 miRNA 之间的关系尚不清楚。在这里，我们证明 FPV 感染通过上调特异性蛋白 1（SP1）的表达，显著改变了 F81 细胞中细胞 miR-92a-1-5p 的表达。此外，我们观察到 miR-92a-1-5p 通过靶向负调控 NF-κB 信号并抑制宿主细胞中 FPV 复制的细胞因子信号转导抑制物 5（SOCS5）来增强干扰素（IFN-α/β）的表达。这些发现表明 miR-92a-1-5p 在宿主抵抗 FPV 感染的防御中发挥着关键作用。
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