Henan Key Laboratory of TCM Syndrome and Prescription in Signaling, Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling, Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.
Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.
Talanta. 2023 Jan 15;252:123775. doi: 10.1016/j.talanta.2022.123775. Epub 2022 Aug 8.
Alkaline phosphatase (ALP) is a significant hydrolase enzyme found in living organisms, and the dysregulation of its physiological activity has been correlated with a variety of diseases. Exploring the activity of ALP has important implications for biomedical research and clinical diagnosis. Accordingly, we have developed a novel, highly sensitive electrochemical biosensor for the analysis of ALP. Based on photoinduced atom transfer radical polymerisation (photoATRP), this strategy combined a fabricated biosensor with hydrolysate produced by the hydrolysis of O-phosphoethanolamine by ALP. Furthermore, for signal amplification, photoATRP synthesises uses polymers with plentiful binding sites for ferrocenylmethyl methacrylate, and by using a photoredox catalyst under blue light irradiation to perform this without the need for copper complexes, it is beneficial for environmental protection compared to traditional atom transfer radical polymerisation (ATRP). The biosensor had a linear range of 10-150 mU·mL, with R = 0.998, and detection limits as low as 2.12 mU·mL. Moreover, by exhibiting outstanding selectivity and interference resistance in human serum samples, this sensor has great potential for practical applications.
碱性磷酸酶（ALP）是一种存在于生物体内的重要水解酶，其生理活性的失调与多种疾病有关。探索 ALP 的活性对生物医学研究和临床诊断具有重要意义。因此，我们开发了一种用于分析 ALP 的新型高灵敏度电化学生物传感器。该策略基于光诱导原子转移自由基聚合（photoATRP），将制备的生物传感器与 ALP 水解 O-磷酸乙醇胺产生的水解产物结合在一起。此外，为了进行信号放大，photoATRP 合成使用具有丰富结合位点的聚合物，用于 ferrocenylmethyl methacrylate，并且在蓝光照射下使用光氧化还原催化剂进行聚合，无需铜配合物，与传统的原子转移自由基聚合（ATRP）相比，更有利于环境保护。该生物传感器的线性范围为 10-150 mU·mL，R=0.998，检测限低至 2.12 mU·mL。此外，该传感器在人血清样本中表现出出色的选择性和抗干扰能力，具有很大的实际应用潜力。
