梅奥肠杆菌角蛋白酶高效降解蛋壳膜。
High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis.
机构信息
Institute of Biological Chemistry, Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei, 11529, Taiwan.
Graduate Institute of Biomedical Sciences, China Medical University, No. 91, Hsueh-Shih Road, Taichung, 40402, Taiwan.
出版信息
Sci Rep. 2022 Aug 29;12(1):14684. doi: 10.1038/s41598-022-18474-4.
Eggshell membrane (ESM), a plentiful biological waste, consists of collagen-like proteins and glycosaminoglycans (GAGs) such as hyaluronic acid (HA). Here we used a keratinase (oeMtaker)-mediated system to decompose ESM. The best reaction condition was established by incubating the solution containing oeMtaker, sodium sulfite, and ESM with a weight ratio of 1:120:600. ESM enzymatic hydrolysate (ESM-EH) showed a high proportion of essential amino acids and type X collagen peptides with 963-2259 Da molecular weights. The amounts of GAGs and sulfated GAGs in ESM-EH were quantified as 6.4% and 0.7%, respectively. The precipitated polysaccharides with an average molecular weight of 1300-1700 kDa showed an immunomodulatory activity by stimulating pro-inflammatory cytokines (IL-6 and TNF-α) production. In addition, a microorganism-based system was established to hydrolyze ESM by Meiothermus taiwanensis WR-220. The amounts of GAGs and sulfated GAGs in the system were quantified as 0.9% and 0.1%, respectively. Based on our pre-pilot tests, the system shows great promise in developing into a low-cost and high-performance process. These results indicate that the keratinase-mediated system could hydrolyze ESM more efficiently and produce more bioactive substances than ever for therapeutical applications and dietary supplements.
蛋壳膜（ESM）是一种丰富的生物废料，由类似于胶原蛋白的蛋白质和糖胺聚糖（GAG）组成，如透明质酸（HA）。在这里，我们使用角蛋白酶（oeMtaker）介导的系统来分解 ESM。通过在含有 oeMtaker、亚硫酸钠和 ESM 的溶液中以 1:120:600 的重量比进行孵育，确定了最佳反应条件。ESM 酶解产物（ESM-EH）表现出高比例的必需氨基酸和类型 X 胶原蛋白肽，分子量为 963-2259 Da。ESM-EH 中的 GAG 和硫酸化 GAG 含量分别定量为 6.4%和 0.7%。沉淀的多糖分子量平均为 1300-1700 kDa，具有刺激促炎细胞因子（IL-6 和 TNF-α）产生的免疫调节活性。此外，还建立了一种基于微生物的系统，通过 Meiothermus taiwanensis WR-220 水解 ESM。该系统中 GAG 和硫酸化 GAG 的含量分别定量为 0.9%和 0.1%。根据我们的初步试验，该系统在开发低成本、高性能工艺方面具有很大的应用前景。这些结果表明，角蛋白酶介导的系统可以更有效地水解 ESM，并产生更多的生物活性物质，用于治疗应用和膳食补充剂。
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