Department of Tropical Diseases of the Second Affiliated Hospital, Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Control of Tropical Diseases, School of Tropical Medicine, Hainan Medical University, Haikou, Hainan, 571199, People's Republic of China.
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Mikrochim Acta. 2022 Aug 30;189(9):355. doi: 10.1007/s00604-022-05402-6.
A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) composed of cobalt-doped metal-organic frameworks (Co-MOF), gold nanoparticles (AuNP), and N-(4-aminobutyl)-N-(ethylisoluminol) (ABEI) is reported. Co-MOF@AuNP@ABEI exhibits high synergistic and zero-distance catalytic properties, which are beneficial to the improvement of the detection sensitivity of an electrochemiluminescent (ECL) biosensor. After coupling with the ECL system and 3D magnetic walking nanomachine amplification strategy, the Co-MOF@AuNP@ABEI can achieve an ultrasensitive ECL assay of Burkholderia pseudomallei with the limit of detection (LOD) of 60.3 aM, which is 2 and 4 orders of magnitude lower than individual ECL system without the nanomachine (4.97 fM) and individual walking nanomachine (340 fM), and superior to the pathogenic bacteria analyses in the previous report. Moreover, the LOD of the proposed ECL detection system for the determination of B. pseudomallei in serum sample was as low as 9.0 CFU mL. The relative standard deviations (RSD) of ECL intensity for the detection of five B. pseudomallei-spiked serum samples were 4.02%, 0.84%, 0.84%, 1.55%, and 0.21%, respectively. The recoveries of the ECL biosensor for the detection of B. pseudomallei DNA-spiked serum samples were 93.63 ~ 107.83%. Therefore, this work demonstrated that the developed multifunctional catalytic nanomaterial with synergistic and zero-distance catalytic properties can be used as excellent ECL signal reporter to improve the detection sensitivity of ECL biosensor.
报道了一种由钴掺杂金属有机骨架（Co-MOF）、金纳米粒子（AuNP）和 N-（4-氨基丁基）-N-（乙基异鲁米诺）（ABEI）组成的多功能催化纳米材料（Co-MOF@AuNP@ABEI）。Co-MOF@AuNP@ABEI 表现出高协同和零距离催化特性，有利于提高电致化学发光（ECL）生物传感器的检测灵敏度。通过与 ECL 系统和 3D 磁性行走纳米机器放大策略结合，Co-MOF@AuNP@ABEI 可以实现对伯克霍尔德菌的超灵敏 ECL 分析，检测限（LOD）低至 60.3 aM，比没有纳米机器（4.97 fM）和单个行走纳米机器（340 fM）的单个 ECL 系统低 2 个和 4 个数量级，优于以前的报道中的病原菌分析。此外，该 ECL 检测系统用于血清样品中假鼻疽伯克霍尔德菌的测定的检出限低至 9.0 CFU mL。用于检测五个假鼻疽伯克霍尔德菌加标血清样品的 ECL 强度的相对标准偏差（RSD）分别为 4.02%、0.84%、0.84%、1.55%和 0.21%。用于检测假鼻疽伯克霍尔德菌 DNA 加标血清样品的 ECL 生物传感器的回收率为 93.63%~107.83%。因此，这项工作表明，具有协同和零距离催化特性的多功能催化纳米材料可用作优异的 ECL 信号报告器，以提高 ECL 生物传感器的检测灵敏度。
