Programa de Pós-Graduação em Ciências Agrárias, Instituto Federal de Educação, Ciência e Tecnologia Goiano (IF Goiano, Campus Rio Verde), Rodovia Sul Goiana, Km 01, Zona Rural, Rio Verde, GO, 75901-970, Brazil.
Instituto Federal de Sergipe (Campus Itabaiana), Avenida Padre Airton Gonçalves Lima, 1140 São Cristóvão, Itabaiana, 49500-543, Brazil.
Sci Rep. 2022 Aug 29;12(1):14702. doi: 10.1038/s41598-022-19126-3.
The soybean looper, Chrysodeixis includens, is a primary soybean pest that reduces crop productivity. This work examined control of C. includens populations with methanolic extract of Serjania erecta, a native Cerrado plant, while minimizing risks to pollinators, natural enemies and the environment. Serjania erecta specimens were collected, identified, and subjected to methanol extraction. Bioassays were performed using newly hatched and second-instar caterpillars and different extract concentrations on the diet surface to obtain IC values. Two replicates, containing 10 caterpillars, were established in triplicate. The IC values were 4.15 and 6.24 mg of extract mL for first-instar and second-instar caterpillars, respectively. These growth inhibition results informed the extract concentrations assessed in subsequent development inhibition assays, in which the pupal weight was higher under the control than under the treatments. Extract treatments increased the duration of the larval, pupal and total development. The potential of different concentrations of S. erecta extract to inhibit the enzymes carboxylesterases was also evaluated. Carboxylesterases activity decreased by 41.96 and 43.43% at 7.8 and 15.6 μg mL extract, respectively. At 31.3 μg mL extract, enzymatic activity was not detected. Overall, S. erecta leaf methanolic extract showed inhibitory potential against carboxylesterases.
大豆尺蠖（Chrysodeixis includens）是一种主要的大豆害虫，会降低作物的生产力。本研究旨在探讨利用 Serjania erecta（一种原产于塞拉多的植物）的甲醇提取物来控制 C.includens 种群，同时最大限度地降低对传粉媒介、天敌和环境的风险。采集、鉴定了 Serjania erecta 标本，并进行了甲醇提取。使用新孵化的和第二龄幼虫进行生物测定，并在饮食表面上使用不同的提取物浓度来获得 IC 值。建立了两个含有 10 只幼虫的重复实验，每个实验重复三次。第一龄和第二龄幼虫的 IC 值分别为 4.15 和 6.24mg 提取物 mL。这些生长抑制结果为后续发育抑制试验中评估的提取物浓度提供了信息，在控制组中蛹重高于处理组。提取物处理延长了幼虫、蛹和总发育时间。还评估了不同浓度的 S.erecta 提取物对抑制羧酸酯酶的潜力。在 7.8 和 15.6μg mL 提取物时，羧酸酯酶活性分别降低了 41.96%和 43.43%。在 31.3μg mL 提取物时，未检测到酶活性。总体而言，S.erecta 叶甲醇提取物对羧酸酯酶具有抑制潜力。
