School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding 071002, China.
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
J Agric Food Chem. 2022 Sep 14;70(36):11419-11428. doi: 10.1021/acs.jafc.2c03618. Epub 2022 Aug 30.
Rapid evolution of resistance in crop pests to (Bt) products threatens their widespread use, especially as pests appear to develop resistance through a range of different physiological adaptations. With such a diverse range of mechanisms reported, researchers have resorted to multi-omic approaches to understand the molecular basis of resistance. Such approaches generate a lot of data making it difficult to establish where causal links between physiological changes and resistance exist. In this study, a combination of RNA-Seq and iTRAQ was used with a strain of diamondback moth, (L.), whose resistance mechanism is well understood. While some of the causal molecular changes in the resistant strain were detected, other previously verified changes were not detected. We suggest that while multi-omic studies have use in validating a proposed resistance mechanism, they are of limited value in identifying such a mechanism in the first place.
作物害虫对 （Bt） 产品的抗性迅速进化，这威胁到它们的广泛使用，尤其是因为害虫似乎通过一系列不同的生理适应来产生抗性。由于报告了如此多样化的机制，研究人员求助于多组学方法来了解抗性的分子基础。这些方法产生了大量的数据，使得很难确定生理变化和抗性之间的因果关系。在这项研究中，使用 RNA-Seq 和 iTRAQ 与一种小菜蛾（L.）的抗性品系相结合，其抗性机制得到了很好的理解。虽然在抗性品系中检测到了一些因果分子变化，但其他先前验证的变化则没有检测到。我们认为，虽然多组学研究在验证提出的抗性机制方面具有用途，但它们在确定抗性机制方面的价值有限。
