Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Department of Medical Science, Amnatcharoen Campus, Mahidol University, Amnat Charoen, 37000, Thailand; Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Talanta. 2023 Jan 15;252:123852. doi: 10.1016/j.talanta.2022.123852. Epub 2022 Aug 17.
Iron-tannic nanoparticles were used as a new adsorbent for dispersive solid phase extraction (DSPE) synergized with cloud point extraction (CPE) to enrich four tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) prior to high-performance liquid chromatographic determination. DSPE and CPE were performed simultaneously in a one-pot co-extraction to form iron-tannic nanoparticles in-situ and pre-concentrate the tetracyclines. The parameters affecting the extraction efficiency were investigated. Using the optimal parameters, linear calibrations ranging from 2.63 to 1000 ng mL were obtained, with determination coefficients greater than 0.996. The limit of detection was found to be 1.06-3.19 ng mL, while the limit of quantification was 2.63-10.65 ng mL. Precision was expressed as a relative standard deviation of less than 10%. The residues of the four tetracyclines in milk, eggs, honey, chicken liver, and chicken kidney samples were determined by the proposed method. The recoveries ranged from 79.3 to 107.1%. The results indicated that the proposed method was an alternative method for the extraction and pre-concentration of tetracyclines with high extraction and enrichment efficiency. In addition, it promoted rapidity and environmental friendliness.
铁单宁纳米粒子被用作一种新型吸附剂，用于分散固相萃取 (DSPE) 与浊点萃取 (CPE) 协同作用，以在高效液相色谱测定之前富集四种四环素（土霉素、四环素、金霉素和强力霉素）。DSPE 和 CPE 同时在一锅共萃取中进行，以原位形成铁单宁纳米粒子并预浓缩四环素。研究了影响萃取效率的参数。使用最佳参数，从 2.63 到 1000ng/mL 范围内获得了线性校准，相关系数大于 0.996。检测限为 1.06-3.19ng/mL，定量限为 2.63-10.65ng/mL。精密度表示为小于 10%的相对标准偏差。该方法用于牛奶、鸡蛋、蜂蜜、鸡肝和鸡肾样品中四种四环素的残留检测。回收率在 79.3%至 107.1%之间。结果表明，该方法是一种替代方法，用于提取和预浓缩四环素，具有高提取和富集效率。此外，它还促进了快速性和环境友好性。
