Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang 330045, China.
Molecules. 2023 Nov 8;28(22):7475. doi: 10.3390/molecules28227475.
In view of the serious side effects of chlortetracycline (CTC) on the human body, it is particularly important to develop rapid, sensitive, and selective technologies for the detection of CTC in food. In this work, a molecularly imprinted electrochemical sensor with [Fe(CN)] as signal probe was proposed for the highly sensitive and selective detection of CTC. For this purpose, TiO, which acts as an interlayer scaffold, was uniformly grown on the surface of TiCT sheets through a simple two-step calcination process using TiCT as the precursor to effectively avoid the stacking of TiCT layers due to hydrogen bonding and van der Waals forces. This endowed TiO@TiCT with large specific surface, abundant functional sites, and rapid mass transfer. Then, polypyrrole molecularly imprinted polymers (MIPs) with outstanding electrical conductivity were modified on the surface of TiO@TiCT via simple electro-polymerization, where the pyrrole was employed as a polymeric monomer and the CTC provided a source of template molecules. This will not only provide specific recognition sites for CTC, but also facilitate electron transport on the electrode surface. The synergistic effects between TiO@TiCT and polypyrrole MIPs afforded the TiO@TiCT/MIP-based electrochemical sensor excellent detection properties toward CTC, including ultra-low limits of detection (LOD) (0.027 nM), a wide linear range (0.06-1000 nM), and outstanding stability, reproducibility, selectivity, and feasibility in real samples. The results indicate that this strategy is feasible and will broaden the horizon for highly sensitive and selective detection of CTC.
鉴于金霉素（CTC）对人体的严重副作用，开发用于食品中 CTC 快速、灵敏和选择性检测的技术尤为重要。在这项工作中，提出了一种以 [Fe(CN)] 6 3- 为信号探针的分子印迹电化学传感器，用于 CTC 的高灵敏和选择性检测。为此，通过使用 TiCT 作为前体的简单两步煅烧过程，在 TiCT 片的表面上均匀生长 TiO 2 ，作为中间层支架，以有效避免由于氢键和范德华力导致的 TiCT 层堆叠。这使 TiO 2 @TiCT 具有大的比表面积、丰富的功能位点和快速的质量传递。然后，通过简单的电聚合在 TiO 2 @TiCT 表面修饰具有优异导电性的聚吡咯分子印迹聚合物（MIPs），其中吡咯用作聚合单体，CTC 提供模板分子的来源。这不仅为 CTC 提供了特定的识别位点，而且有利于电极表面的电子传递。TiO 2 @TiCT 和聚吡咯 MIPs 的协同作用赋予基于 TiO 2 @TiCT/MIP 的电化学传感器对 CTC 具有优异的检测性能，包括超低检测限（LOD）（0.027 nM）、宽线性范围（0.06-1000 nM）以及出色的稳定性、重现性、选择性和在实际样品中的可行性。结果表明，该策略是可行的，并将拓宽高灵敏度和选择性检测 CTC 的视野。
