Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-6515, United States.
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, United States.
Food Chem. 2023 Jan 15;399:133955. doi: 10.1016/j.foodchem.2022.133955. Epub 2022 Aug 17.
Herbicides atrazine and acetochlor are used in crop production. Because of environmental and health hazards with respective maximum contamination levels of 3 and 20 ng/mL, quantifying these herbicides is important when considering presence in foods and vegetables. We utilized two Pd@Pt nanoparticle-amplified immunoassays, a colorimetric Pd@Pt nanoparticle-linked immunosorbent assay (NLISA) and differential pulse voltammetry (DPV) dependent on catalytic activity of Pd@Pt in a dual-lateral flow immunoassay (dual-LFIA-DPV). We achieved overall recoveries of 88.5-114 % in juice, fruit, and vegetable samples for both immunoassays. The NLISA yielded limits of detection (LODs) of 0.59 and 0.31 µg/kg and the dual-LFIA-DPV 0.27 and 0.51 µg/kg for the two respective species. Results for both immunoassays were validated by high-performance liquid chromatography (HPLC), for all food and drink samples though LODs are compromised when configuring the HPLC for both species with the same chromatogram. We expect Pd@Pt-based immunoassays to prove useful in various fields.
除草剂莠去津和乙草胺用于作物生产。由于各自的最大污染水平为 3 和 20 ng/mL 的环境和健康危害，因此在考虑食品和蔬菜中存在这些除草剂时，对其进行定量分析很重要。我们利用两种 Pd@Pt 纳米粒子放大的免疫分析方法，即比色 Pd@Pt 纳米粒子连接免疫分析（NLISA）和依赖于 Pd@Pt 在双侧向流动免疫分析（dual-LFIA-DPV）中的催化活性的差分脉冲伏安法（DPV）。我们在果汁、水果和蔬菜样品中实现了两种免疫分析方法的总体回收率为 88.5-114%。NLISA 的检测限（LOD）分别为 0.59 和 0.31 µg/kg，dual-LFIA-DPV 分别为 0.27 和 0.51 µg/kg。尽管在为两种物质配置相同的色谱图时会降低 HPLC 的 LOD，但两种免疫分析方法的结果均通过高效液相色谱法（HPLC）进行了验证，适用于所有食品和饮料样品。我们预计基于 Pd@Pt 的免疫分析将在各个领域证明有用。
