Effects of vapor pressure deficit combined with different N levels on tomato seedling anatomy, photosynthetic performance, and N uptake.

Vapor pressure difference (VPD) is the main driving force of plant transpiration and the main factor of greenhouse environment regulation. Nitrogen is the main element of crop growth and development. It is significant to explore the regulation of VPD on nitrogen absorption and its effect on tomato photosynthesis. In this paper, using tomato as material, using an artificial climate chamber, the effect of VPD and nitrogen level coupling on nitrogen absorption and distribution, hydraulic characteristics, and photosynthetic characteristics of tomato was studied and analyzed. The optimal regulation of VPD and nitrogen was analyzed. Studies have shown that appropriately reducing the VPD can promote the absorption of nitrogen by plants. The increased surface area and volume of tomato roots and the increased activity of nitrogen assimilation-related enzymes were beneficial to nitrogen absorption and assimilation. Compared with high VPD (HVPD) plants, the leaf thickness and spongy tissue thickness of low VPD (LVPD) plants decreased, and the palisade/spongy tissue thickness ratio (P/S) increased; Leaf water conductance (K) increased with the increase of nitrogen level. The K at normal and high nitrogen plants increased by 4.00 % and 33.93 %, respectively, compared with HVPD plants of the same nitrogen level (significant difference at high nitrogen level) but significantly decreased at low nitrogen level. The decrease of spongy tissue thickness, the increase of palisade/sponge tissue, and the up-regulation of aquaporin expression were all beneficial to increasing K. Decreasing VPD and increasing nitrogen application under LVPD both increased specific leaf area (SLA). Compared with HVPD treatment, the photosynthetic rate of LVPD-treated plants increased by 7.06 % and 30.48 % at normal and high nitrogen levels, respectively.