Division of Veterinary Parasitology, Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka.
Zootopia Vets Animal Clinic, Nugegoda, Sri Lanka.
Vet Parasitol Reg Stud Reports. 2022 Sep;34:100762. doi: 10.1016/j.vprsr.2022.100762. Epub 2022 Jul 20.
Platynosomum is a digenean trematode causing hepatobiliary disease in cats in tropical and subtropical regions. The presence of Platynosomum species in Sri Lanka has not been previously reported or investigated. In the current study, we report a clinical case of a cat suffering from hepatic and biliary complications. Fine-needle aspiration of the biliary extract revealed a large number of parasite eggs, and the morphological and molecular identification of eggs was carried out. Molecular phylogenetics was performed using the nuclear ribosomal Internal Transcribed Spacer 2 (ITS2) and a portion of a mitochondrially encoded gene; Cytochrome C Oxidase subunit 1 (COX1). Through a combination of history, clinical signs, blood reports, ultrasound scanning, light microscopy of eggs from biliary aspirate and molecular studies, the disease was confirmed as parasitism caused by a Platynosomum like species. The Platynosomum species in Sri Lanka is phylogenetically related to Platynosomum illiciens reported from Costa Rica, Central America. This case emphasizes the importance of future studies in Sri Lanka regarding the prevalence and distribution of Platynosomum among cat populations. Further, the inclusion of feline platynosomiasis in the differential diagnoses list for hepatobiliary diseases is required.
片形属是一种引起热带和亚热带地区猫科动物肝胆疾病的复殖吸虫。以前没有报道或调查过斯里兰卡存在片形属物种。在本研究中，我们报告了一例患有肝胆并发症的猫的临床病例。对胆汁提取液进行细针抽吸，发现大量寄生虫卵，并对卵进行形态学和分子鉴定。使用核核糖体内部转录间隔区 2（ITS2）和一部分线粒体编码基因；细胞色素 C 氧化酶亚基 1（COX1）进行分子系统发育分析。通过病史、临床症状、血液报告、超声扫描、胆汁抽吸物中卵的光镜检查以及分子研究的结合，确认该疾病是由一种类似于 Platynosomum 的物种引起的寄生虫感染。斯里兰卡的 Platynosomum 物种与从中美洲哥斯达黎加报告的 Platynosomum illiciens 在系统发育上相关。该病例强调了未来在斯里兰卡开展关于猫群中 Platynosomum 流行和分布的研究的重要性。此外，需要将猫类片形吸虫病纳入肝胆疾病的鉴别诊断列表中。
