Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca 14853-6401, NY, USA.
Department of Medical Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Vet Parasitol Reg Stud Reports. 2022 Sep;34:100758. doi: 10.1016/j.vprsr.2022.100758. Epub 2022 Jul 5.
A fecal pellet was recovered in an ancient salt mine in Chehrabad located in western Iran (36.55° N, 47.51° E). Based on prior publications showing the success of the salt mine's environment to preserve various life forms, it was decided to try and ascertain whether this faex contained any parasites of paleoparasitologic interest. The rehydration involved placing the pellet in an aqueous solution of 0.5% trisodium phosphate for a week, followed by the examination of aliquots of the entire rehydrated sample on a total of 153 microscope slides. The examination of the rehydrated material revealed the presence of two larval nematodes; there were no eggs or oocysts recovered. The larvae were photographed and measured, and the decision was made not to submit the two larvae to grinding and DNA extraction for molecular diagnostics due to their excellent state of preservation. The larvae were identified as first-stage larvae that appear to represent the genus Muellerius. Herein are reported the finding of two first-stage larvae of Muellerius nematodes (Metastrongyloidea, Protostrongylidae) from the rehydrated fecal pellet collected in a Chehrabad salt mine from the era of the Sasanian Empire.
在伊朗西部的 Chehrabad 古代盐矿中发现了一个粪便颗粒（36.55° N, 47.51° E）。基于先前的出版物表明盐矿环境成功保存了各种生命形式，因此决定尝试确定这个粪便颗粒是否含有任何古寄生虫学感兴趣的寄生虫。再水化涉及将粪便颗粒放置在 0.5%的三磷酸钠水溶液中一周，然后在总共 153 张显微镜载玻片上检查整个再水化样品的等分试样。对再水化材料的检查显示存在两条幼虫线虫；没有回收卵或卵囊。幼虫被拍照和测量，由于其保存状态极佳，决定不将两条幼虫送去研磨和 DNA 提取进行分子诊断。这些幼虫被鉴定为第一阶段幼虫，似乎代表 Muellerius 属。本文报道了在伊朗西部 Chehrabad 盐矿中从萨珊帝国时期采集的再水化粪便颗粒中发现的两条 Muellerius 线虫（圆线虫目，原圆线虫科）的第一阶段幼虫。
