Promotes Crown Root Formation via Cytokinin in .

BACKGROUND

Crown root is the main part of root system, which performs an important role in rice growth and development, especially in nutrition and water assimilation. Previously, we reported negative feedback regulation loop between () and cytokinin by () in rice development.

METHODS

Reverse transcription quantitative RT-PCR analyses was used to analyze the related gene transcript level. Nitrogen and hormone were measured by CHN-Nitrogen analyser and Liquid chromatography mass spectrometer, respectively. Exogenous application of cytokinin and [13C] sucrose labeled stable isotope uptake experiments help us to explain the relationship between OsDOF11 and cytokinin.

RESULTS

We demonstrate the role of in root development. We note that the loss function of displays the reduced crown roots number, low activity of nitrogen assimilation and low content of cytokinin and auxin. The expression level of (), (), , and were decreased in , as well as in mutants ( lines). Through Exogenous application of multiple concentrations of cytokinin as treatment to mutant, lines, and wild type (WT). We found that the crown roots number of plants were rescued as the cytokinin concentration increased gradually from 1 μM to 10 μM, but the effect was weaker in line. And cytokinin inhibited sucrose uptake activity from Murashige-Skoog medium with 3.0% sucrose (MS30) by in rice root.

CONCLUSIONS

promotes crown root formation via cytokinin in . These results provide a physiological basis for further analysis of the function of in rice root development.