PLOD2、上皮-间质转化标志物和癌症干细胞在食管鳞状细胞癌患者中的临床病理意义。

Clinicopathological significances of PLOD2, epithelial-mesenchymal transition markers, and cancer stem cells in patients with esophageal squamous cell carcinoma.

机构信息

Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Department of Ophthalmology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

出版信息

Medicine (Baltimore). 2022 Aug 26;101(34):e30112. doi: 10.1097/MD.0000000000030112.

DOI:10.1097/MD.0000000000030112

PMID:36042592

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9410680/

Abstract

BACKGROUND

To examine the expression level of procollagen-lysine2-oxoglutarate 5-dioxygenase 2 (PLOD2) in esophageal squamous cell carcinoma (ESCC) and analyze its correlation with clinicopathological parameters, in order to explore the mechanism of PLOD2 in regulating invasion and metastasis of ESCC.

METHODS

Immunohistochemistry was used to detect the expression level of PLOD2 in tumor tissues and paired adjacent tissues of 172 patients with ESCC, and the relationship between PLOD2 expression and clinicopathological parameters was analyzed. The deposition of collagen fibers in tumor was detected by Sirius red staining. The correlation between tumor stem cells and epithelial-mesenchymal transition (EMT) markers ZEB1 was analyzed by multivariate logistic regression.

RESULTS

The expression level of PLOD2 in tumor tissues of patients with ESCC (70.35%, 121/172) was significantly higher than that in paired adjacent tissues (29.65%, 51/172; P < .01). The positive expression rate of PLOD2 in ESCC was related to T classification, lymph node metastasis, and pathological tumor node metastasis of a tumor. The expression rates of ZEB1, CD44, and CD133 in ESCC were correlated with T classification, lymph node metastasis and pathological tumor node metastasis. Scarlet red staining showed that collagen fiber deposition in ESCC tissues with high expression of PLOD2 was significantly higher than that in tissues with low expression of PLOD2 (P < .01). A positive correlation was observed between the expression of PLOD2 and CD133, PLOD2 and CD44, and PLOD2 and N-cadherin (P < .01). Moreover, a negative correlation was noted between the expression of PLOD2 and E-cadherin (P < .01). The combined expression of PLOD2 and ZEB1 were independent prognostic factors for the total survival time of patients with ESCC.

CONCLUSION

PLOD2 is highly expressed in ESCC and is closely related to tumor invasion and metastasis. The mechanism of PLOD2 for promoting invasion and metastasis of ESCC may be related to activation of the EMT signaling pathway to promote EMT and tumor stem cell transformation.

摘要

背景

检测赖氨酰氧化酶样蛋白 2（PLOD2）在食管鳞状细胞癌（ESCC）中的表达水平，并分析其与临床病理参数的相关性，以探讨 PLOD2 调节 ESCC 侵袭和转移的机制。

方法

采用免疫组织化学法检测 172 例 ESCC 患者肿瘤组织及配对癌旁组织中 PLOD2 的表达水平，分析 PLOD2 表达与临床病理参数的关系。天狼猩红染色检测肿瘤组织中胶原纤维的沉积。采用多元 logistic 回归分析肿瘤干细胞标志物 CD133 与上皮间质转化（EMT）标志物 ZEB1 的相关性。

结果

ESCC 肿瘤组织中 PLOD2 的表达水平（70.35%，121/172）明显高于配对癌旁组织（29.65%，51/172；P<0.01）。PLOD2 在 ESCC 中的阳性表达率与 T 分期、淋巴结转移和病理肿瘤淋巴结转移有关。ESCC 中 ZEB1、CD44 和 CD133 的表达率与 T 分期、淋巴结转移和病理肿瘤淋巴结转移有关。天狼猩红染色显示，PLOD2 高表达的 ESCC 组织中胶原纤维沉积明显高于 PLOD2 低表达的组织（P<0.01）。PLOD2 与 CD133、PLOD2 与 CD44 以及 PLOD2 与 N-钙黏蛋白呈正相关（P<0.01）。此外，PLOD2 与 E-钙黏蛋白呈负相关（P<0.01）。PLOD2 与 ZEB1 的联合表达是 ESCC 患者总生存时间的独立预后因素。