Veraguas-Dávila Daniel, Saéz-Ruíz Darling, Álvarez María Consuelo, Saravia Fernando, Castro Fidel Ovidio, Rodríguez-Alvarez Lleretny
Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Chillán, Chile.
Zygote. 2022 Dec;30(6):841-848. doi: 10.1017/S096719942200034X. Epub 2022 Aug 31.
Domestic cat embryos generated by fertilization (IVF) and cultured without the zona pellucida have a reduced implantation capacity after embryo transfer at the blastocyst stage. The objective of this study was to evaluate the expression of trophectoderm markers in domestic cat blastocysts cultured without the zona pellucida. Two experimental groups were selected: (1) domestic cat embryos generated by IVF and cultured normally (zona intact group, ZI); and (2) domestic cat embryos generated by IVF and cultured without a zona pellucida (zona-free group, ZF). In the ZF group, the zona pellucida of the presumptive zygote was removed and these were cultured using the well of the well (WOW) system. culture was carried out for 7 days. The cleavage, morula and blastocyst rates were estimated. Finally, the relative expression levels of the trophectoderm markers , , and , the cell adhesion marker and the apoptosis marker were evaluated by RT-qPCR in the blastocysts. The Wilcoxon test was used to evaluate differences ( < 0.05). No differences were observed in the cleavage, morula and blastocyst rates between the ZF and ZI groups. No differences were found in the expression of , , and between groups. The expression of and was higher in ZF blastocysts than in ZI blastocysts. In conclusion, the culture without the zona pellucida generates an overexpression of and in the domestic cat blastocysts. More studies are needed to confirm if this overexpression might affect development.
通过体外受精（IVF）产生并在无透明带条件下培养的家猫胚胎，在囊胚阶段进行胚胎移植后，其着床能力降低。本研究的目的是评估在无透明带条件下培养的家猫囊胚中滋养外胚层标志物的表达。选择了两个实验组：（1）通过IVF产生并正常培养的家猫胚胎（完整透明带组，ZI）；（2）通过IVF产生并在无透明带条件下培养的家猫胚胎（无透明带组，ZF）。在ZF组中，去除推定合子的透明带，并使用逐孔（WOW）系统进行培养。培养7天。估计卵裂、桑葚胚和囊胚率。最后，通过RT-qPCR评估囊胚中滋养外胚层标志物、、和、细胞粘附标志物以及凋亡标志物的相对表达水平。使用Wilcoxon检验评估差异（<0.05）。ZF组和ZI组之间在卵裂、桑葚胚和囊胚率方面未观察到差异。两组之间在、、和的表达上未发现差异。ZF囊胚中及的表达高于ZI囊胚。总之，无透明带培养导致家猫囊胚中及的过表达。需要更多研究来证实这种过表达是否可能影响发育。
