Lin Jing-Ying, Tsai Bruce Chi-Kang, Kao Hui-Chuan, Chiang Chien-Yi, Chen Yun-An, Chen William Shao-Tsu, Ho Tsung-Jung, Yao Chun-Hsu, Kuo Wei-Wen, Huang Chih-Yang
Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung, Taiwan.
Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
Probiotics Antimicrob Proteins. 2023 Oct;15(5):1287-1297. doi: 10.1007/s12602-022-09982-w. Epub 2022 Aug 31.
Diabetes-related brain complications have been reported in clinical patients and experimental models. The objective of the present study was to investigate the neuroprotective mechanisms of Lactobacillus reuteri GMNL-263 in streptozotocin (STZ)-induced diabetic rats. In this study, three different groups, namely control group, STZ-induced (55 mg/kg streptozotocin intraperitoneally) diabetic rats (DM), and DM rats treated with Lactobacillus reuteri GMNL-263 (1 × 10 CFU/rat/day), were utilized to study the protective effect of GMNL-263 in the hippocampus of STZ-induced diabetic rats. The results demonstrated that GMNL-263 attenuated diabetes-induced hippocampal damage by enhancing the cell survival pathways and repressing both inflammatory and apoptotic pathways. Histopathological analysis revealed that GMNL-263 prevented structural changes in the hippocampus in the DM group and decreased the level of inflammation and apoptosis in the hippocampus of DM rats. The IGF1R cell survival signaling pathway also improved after GMNL-263 treatment. These results indicate that probiotic GMNL-263 exerts beneficial effects in the brain of diabetic rats and has potential ability for clinical application.
临床患者和实验模型中均已报道了糖尿病相关的脑部并发症。本研究的目的是探讨罗伊氏乳杆菌GMNL-263对链脲佐菌素（STZ）诱导的糖尿病大鼠的神经保护机制。在本研究中，使用了三个不同的组，即对照组、STZ诱导（腹腔注射55 mg/kg链脲佐菌素）的糖尿病大鼠（DM）以及用罗伊氏乳杆菌GMNL-263（1×10 CFU/大鼠/天）治疗的DM大鼠，来研究GMNL-263对STZ诱导的糖尿病大鼠海马体的保护作用。结果表明，GMNL-263通过增强细胞存活途径以及抑制炎症和凋亡途径，减轻了糖尿病诱导的海马体损伤。组织病理学分析显示，GMNL-263可防止DM组海马体的结构变化，并降低DM大鼠海马体中的炎症和凋亡水平。GMNL-263治疗后，IGF1R细胞存活信号通路也得到改善。这些结果表明，益生菌GMNL-263对糖尿病大鼠的脑部具有有益作用，并且具有临床应用的潜在能力。
